LOCUS BBa_J24770 9935 bp DNA linear 14-May-2024 DEFINITION 90. 67(BBa_J24748) == 54(BBa_J24734) ACCESSION BBa_J24770 VERSION BBa_J24770.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1143 /label=BBa_C0080 misc_feature 1181..1184 /label=conserved RBS 1177..1188 /label=BBa_B0034 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1854 /label=BBa_C0040 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 misc_feature 2842..2845 /label=conserved RBS 2836..2849 /label=BBa_B0031 CDS 2856..3731 /label=araC misc_feature 3732..3764 /label=LVA misc_feature 3771..3795 /label=Help:Barcodes CDS 2856..3770 /label=BBa_C0080 misc_feature 3810..3813 /label=conserved RBS 3804..3817 /label=BBa_B0031 misc_feature 4913..4951 /label=LVA CDS 3824..4951 /label=lacI-LVA misc_feature 3824..4951 /label=BBa_C0012 misc_feature 4952..4976 /label=Help:Barcodes CDS 3824..4951 /label=BBa_C0012 misc_feature 4989..4992 /label=conserved RBS 4985..4996 /label=BBa_B0034 CDS 5003..5764 /label=YFP (LVA) misc_feature 5759..5764 /label=2 misc_feature 5720..5758 /label=SsrA misc_feature 5244..5244 /label=A (Q->L) misc_feature 5003..5764 /label=BBa_E0032 misc_feature 5003..5764 /label=BBa_E0032 stem_loop 5784..5827 /label=stem_loop misc_feature 5773..5852 /label=BBa_B0010 terminator 5773..5852 /label=BBa_B0010 stem_loop 5868..5887 /label=T7 TE misc_feature 5894..5894 /label=stop polyA_site 5888..5901 /label=polya misc_feature 5861..5901 /label=BBa_B0012 terminator 5861..5901 /label=BBa_B0012 promoter 6989..7119 /label=Pbad promoter 6952..7023 /label=PC misc_feature 5910..6988 /label=AraC promoter 5910..7119 /label=BBa_I0500 misc_feature 7132..7135 /label=conserved RBS 7128..7139 /label=BBa_B0034 misc_feature 8235..8273 /label=LVA CDS 7146..8273 /label=lacI-LVA misc_feature 7146..8273 /label=BBa_C0012 misc_feature 8274..8298 /label=Help:Barcodes CDS 7146..8273 /label=BBa_C0012 RBS 8307..8319 /label=RBS-3\Weak RBS 8313..8316 /label=RBS misc_feature 8307..8319 /label=BBa_B0032 RBS 8307..8319 /label=BBa_B0032 CDS 8329..8945 /label=tetR misc_feature 8946..8979 /label=SsrA misc_feature 8986..9010 /label=Help:Barcodes CDS 8326..8985 /label=BBa_C0040 misc_feature 9023..9026 /label=conserved RBS 9019..9030 /label=BBa_B0034 CDS 9037..9798 /label=CFP (LVA) misc_feature 9755..9792 /label=SsrA misc_feature 9793..9798 /label=2 misc_feature 9105..9105 /label=A misc_feature 9037..9798 /label=BBa_E0022 misc_feature 9037..9798 /label=BBa_E0022 stem_loop 9818..9861 /label=stem_loop misc_feature 9807..9886 /label=BBa_B0010 terminator 9807..9886 /label=BBa_B0010 stem_loop 9902..9921 /label=T7 TE misc_feature 9928..9928 /label=stop polyA_site 9922..9935 /label=polya misc_feature 9895..9935 /label=BBa_B0012 terminator 9895..9935 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagtcaca caggaaacct actagatggc tgaagcgcaa aatgatcccc 2881 tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca 2941 atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc 3001 tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg 3061 gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc 3121 gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta 3181 actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc 3241 atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg 3301 agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg 3361 agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc 3421 acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt 3481 cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg 3541 accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg 3601 tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca 3661 ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg 3721 tcaagttgtc agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 3781 gctagtgtag atcactacta gagtcacaca ggaaacctac tagatggtga atgtgaaacc 3841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 3901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 3961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 4921 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 4981 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 5041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 5101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 5161 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 5221 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 5281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 5341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 5401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 5461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 5521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 5581 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 5641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 5701 catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta 5761 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 5821 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 5881 gtgggccttt ctgcgtttat atactagagt tatgacaact tgacggctac atcattcact 5941 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc 6001 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc 6061 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag 6121 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca 6181 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga 6241 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg 6301 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc 6361 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca 6421 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag 6481 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga 6541 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat 6601 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct 6661 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt 6721 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct 6781 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc 6841 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg 6901 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa 6961 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc 7021 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa 7081 ctctctactg tttctccata cccgtttttt tgggctagct actagagaaa gaggagaaat 7141 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 7201 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 7261 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 7321 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 7381 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 7441 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 7501 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 7561 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 7621 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 7681 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 7741 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 7801 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 7861 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 7921 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 7981 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 8041 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 8101 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 8161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 8221 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 8281 agtgctagtg tagatctcta ctagagtcac acaggaaagt actagatgtc cagattagat 8341 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta 8401 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta 8461 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact 8521 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt 8581 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct 8641 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt 8701 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta 8761 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt 8821 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc 8881 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt 8941 gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag 9001 tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 9061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 9121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 9181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 9241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 9301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 9361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 9421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 9481 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 9541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 9601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 9661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 9721 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 9781 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 9841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 9901 tggctcacct tcgggtgggc ctttctgcgt ttata //