LOCUS BBa_J24777 9934 bp DNA linear 14-May-2024 DEFINITION 95. 66(BBa_J24747) == 59(BBa_J24739) ACCESSION BBa_J24777 VERSION BBa_J24777.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 RBS 1177..1189 /label=RBS-3\Weak RBS 1183..1186 /label=RBS misc_feature 1177..1189 /label=BBa_B0032 RBS 1177..1189 /label=BBa_B0032 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1177..1880 /label=BBa_J24687 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1177..2766 /label=BBa_J24706 misc_feature 1..2766 /label=BBa_J24715 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 misc_feature 2843..2846 /label=conserved RBS 2837..2850 /label=BBa_B0031 CDS 2857..3732 /label=araC misc_feature 3733..3765 /label=LVA misc_feature 3772..3796 /label=Help:Barcodes CDS 2857..3796 /label=BBa_C0080 misc_feature 2775..3796 /label=BBa_J24694 misc_feature 3809..3812 /label=conserved RBS 3805..3816 /label=BBa_B0034 misc_feature 4912..4950 /label=LVA CDS 3823..4950 /label=lacI-LVA misc_feature 3823..4950 /label=BBa_C0012 misc_feature 4951..4975 /label=Help:Barcodes CDS 3823..4950 /label=BBa_C0012 misc_feature 3805..4975 /label=BBa_J24685 misc_feature 4988..4991 /label=conserved RBS 4984..4995 /label=BBa_B0034 CDS 5002..5763 /label=YFP (LVA) misc_feature 5758..5763 /label=2 misc_feature 5719..5757 /label=SsrA misc_feature 5243..5243 /label=A (Q->L) misc_feature 5002..5763 /label=BBa_E0032 misc_feature 5002..5763 /label=BBa_E0032 stem_loop 5783..5826 /label=stem_loop misc_feature 5772..5851 /label=BBa_B0010 terminator 5772..5851 /label=BBa_B0010 stem_loop 5867..5886 /label=T7 TE misc_feature 5893..5893 /label=stop polyA_site 5887..5900 /label=polya misc_feature 5860..5900 /label=BBa_B0012 terminator 5860..5900 /label=BBa_B0012 misc_feature 4984..5900 /label=BBa_J24704 misc_feature 3805..5900 /label=BBa_J24713 misc_feature 2775..5900 /label=BBa_J24725 misc_feature 1..5900 /label=BBa_J24747 promoter 6988..7118 /label=Pbad promoter 6951..7022 /label=PC misc_feature 5909..6987 /label=AraC promoter 5909..7118 /label=BBa_I0500 misc_feature 7131..7134 /label=conserved RBS 7127..7138 /label=BBa_B0034 misc_feature 8234..8272 /label=LVA CDS 7145..8272 /label=lacI-LVA misc_feature 7145..8272 /label=BBa_C0012 misc_feature 8273..8297 /label=Help:Barcodes CDS 7145..8272 /label=BBa_C0012 misc_feature 7127..8297 /label=BBa_J24685 misc_feature 5909..8297 /label=BBa_J24699 RBS 8306..8318 /label=RBS-3\Weak RBS 8312..8315 /label=RBS misc_feature 8306..8318 /label=BBa_B0032 RBS 8306..8318 /label=BBa_B0032 CDS 8328..8944 /label=tetR misc_feature 8945..8978 /label=SsrA misc_feature 8985..9009 /label=Help:Barcodes CDS 8325..9009 /label=BBa_C0040 misc_feature 8306..9009 /label=BBa_J24687 misc_feature 9022..9025 /label=conserved RBS 9018..9029 /label=BBa_B0034 CDS 9036..9797 /label=CFP (LVA) misc_feature 9754..9791 /label=SsrA misc_feature 9792..9797 /label=2 misc_feature 9104..9104 /label=A misc_feature 9036..9797 /label=BBa_E0022 misc_feature 9036..9797 /label=BBa_E0022 stem_loop 9817..9860 /label=stem_loop misc_feature 9806..9885 /label=BBa_B0010 terminator 9806..9885 /label=BBa_B0010 stem_loop 9901..9920 /label=T7 TE misc_feature 9927..9927 /label=stop polyA_site 9921..9934 /label=polya misc_feature 9894..9934 /label=BBa_B0012 terminator 9894..9934 /label=BBa_B0012 misc_feature 9018..9934 /label=BBa_J24703 misc_feature 8306..9934 /label=BBa_J24709 misc_feature 5909..9934 /label=BBa_J24739 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaacc tactagatgg ctgaagcgca aaatgatccc 2881 ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc gattgaggcc 2941 aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat 3001 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ctgccgaccg 3061 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct 3121 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt 3181 aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc gcaccagccg 3241 catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg gcgctattcg 3301 gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga agcgattaac 3361 gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta catcagcgat 3421 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cctgtcgccg 3481 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag 3541 gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc tatcgccacc 3601 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt taaaaaatgc 3661 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc 3721 gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 3781 tgctagtgta gatcactact agagaaagag gagaaatact agatggtgaa tgtgaaacca 3841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 3961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 4021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 4081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 4141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 4201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 4261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 4321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 4381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 4441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 4501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 4621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 4681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 4741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 4801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac 4921 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 4981 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 5041 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 5101 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 5161 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 5221 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 5281 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 5341 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 5401 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 5461 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 5521 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 5581 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 5641 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 5701 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 5761 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5821 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5881 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt 5941 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc 6001 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc 6061 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga 6121 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat 6181 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac 6241 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc 6301 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc 6361 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat 6421 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt 6481 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac 6541 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt 6601 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt 6661 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta 6721 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt 6781 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca 6841 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc 6901 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa 6961 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 7021 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 7081 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagaaag aggagaaata 7141 ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc 7201 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga 7261 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact 7321 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc 7381 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt 7441 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc 7501 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt 7561 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat 7621 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc 7681 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct 7741 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg 7801 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg 7861 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc 7921 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac 7981 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct 8041 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa 8101 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac 8161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 8221 ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata 8281 gtgctagtgt agatctctac tagagtcaca caggaaagta ctagatgtcc agattagata 8341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 8401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 8461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 8521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 8581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 8641 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 8701 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 8761 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 8821 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 8881 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 8941 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 9001 gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 9061 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 9121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 9181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg 9241 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 9301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 9361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 9421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc 9481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc 9541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 9601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 9661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 9721 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 9781 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 9841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 9901 ggctcacctt cgggtgggcc tttctgcgtt tata //