LOCUS BBa_J24780 9936 bp DNA linear 15-May-2024 DEFINITION 98. 73(BBa_J24754) == 53(BBa_J24733) ACCESSION BBa_J24780 VERSION BBa_J24780.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 RBS 1176..1188 /label=RBS-3\Weak RBS 1182..1185 /label=RBS misc_feature 1176..1188 /label=BBa_B0032 RBS 1176..1188 /label=BBa_B0032 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1176..1879 /label=BBa_J24687 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1176..2765 /label=BBa_J24706 misc_feature 1..2765 /label=BBa_J24718 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 RBS 2836..2848 /label=RBS-3\Weak RBS 2842..2845 /label=RBS misc_feature 2836..2848 /label=BBa_B0032 RBS 2836..2848 /label=BBa_B0032 CDS 2855..3730 /label=araC misc_feature 3731..3763 /label=LVA misc_feature 3770..3794 /label=Help:Barcodes CDS 2855..3794 /label=BBa_C0080 misc_feature 2774..3794 /label=BBa_J24695 misc_feature 3809..3812 /label=conserved RBS 3803..3816 /label=BBa_B0031 misc_feature 4912..4950 /label=LVA CDS 3823..4950 /label=lacI-LVA misc_feature 3823..4950 /label=BBa_C0012 misc_feature 4951..4975 /label=Help:Barcodes CDS 3823..4950 /label=BBa_C0012 misc_feature 3803..4975 /label=BBa_J24683 misc_feature 4988..4991 /label=conserved RBS 4984..4995 /label=BBa_B0034 CDS 5002..5763 /label=YFP (LVA) misc_feature 5758..5763 /label=2 misc_feature 5719..5757 /label=SsrA misc_feature 5243..5243 /label=A (Q->L) misc_feature 5002..5763 /label=BBa_E0032 misc_feature 5002..5763 /label=BBa_E0032 stem_loop 5783..5826 /label=stem_loop misc_feature 5772..5851 /label=BBa_B0010 terminator 5772..5851 /label=BBa_B0010 stem_loop 5867..5886 /label=T7 TE misc_feature 5893..5893 /label=stop polyA_site 5887..5900 /label=polya misc_feature 5860..5900 /label=BBa_B0012 terminator 5860..5900 /label=BBa_B0012 misc_feature 4984..5900 /label=BBa_J24704 misc_feature 3803..5900 /label=BBa_J24711 misc_feature 2774..5900 /label=BBa_J24726 misc_feature 1..5900 /label=BBa_J24754 promoter 6988..7118 /label=Pbad promoter 6951..7022 /label=PC misc_feature 5909..6987 /label=AraC promoter 5909..7118 /label=BBa_I0500 misc_feature 7133..7136 /label=conserved RBS 7127..7140 /label=BBa_B0031 misc_feature 8236..8274 /label=LVA CDS 7147..8274 /label=lacI-LVA misc_feature 7147..8274 /label=BBa_C0012 misc_feature 8275..8299 /label=Help:Barcodes CDS 7147..8274 /label=BBa_C0012 misc_feature 7127..8299 /label=BBa_J24683 misc_feature 5909..8299 /label=BBa_J24697 RBS 8308..8320 /label=RBS-3\Weak RBS 8314..8317 /label=RBS misc_feature 8308..8320 /label=BBa_B0032 RBS 8308..8320 /label=BBa_B0032 CDS 8330..8946 /label=tetR misc_feature 8947..8980 /label=SsrA misc_feature 8987..9011 /label=Help:Barcodes CDS 8327..9011 /label=BBa_C0040 misc_feature 8308..9011 /label=BBa_J24687 misc_feature 9024..9027 /label=conserved RBS 9020..9031 /label=BBa_B0034 CDS 9038..9799 /label=CFP (LVA) misc_feature 9756..9793 /label=SsrA misc_feature 9794..9799 /label=2 misc_feature 9106..9106 /label=A misc_feature 9038..9799 /label=BBa_E0022 misc_feature 9038..9799 /label=BBa_E0022 stem_loop 9819..9862 /label=stem_loop misc_feature 9808..9887 /label=BBa_B0010 terminator 9808..9887 /label=BBa_B0010 stem_loop 9903..9922 /label=T7 TE misc_feature 9929..9929 /label=stop polyA_site 9923..9936 /label=polya misc_feature 9896..9936 /label=BBa_B0012 terminator 9896..9936 /label=BBa_B0012 misc_feature 9020..9936 /label=BBa_J24703 misc_feature 8308..9936 /label=BBa_J24709 misc_feature 5909..9936 /label=BBa_J24733 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaagta ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagtcaca caggaaagta ctagatggct gaagcgcaaa atgatcccct 2881 gctgccggga tactcgttta acgcccatct ggtggcgggt ttaacgccga ttgaggccaa 2941 tggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata ttctcaatct 3001 caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgtct gccgaccggg 3061 tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc cggaggctcg 3121 cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg aatggcttaa 3181 ctggccgtca atatttgcca atacgggttt ctttcgcccg gatgaagcgc accagccgca 3241 tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc gctattcgga 3301 gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag cgattaacga 3361 gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca tcagcgatca 3421 cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgcc tgtcgccgtc 3481 gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga 3541 ccaacgcatc agccaggcga agctgctttt gagcactacc cggatgccta tcgccaccgt 3601 cggtcgcaat gttggttttg acgatcaact ctatttctcg cgagtattta aaaaatgcac 3661 cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt 3721 caagttgtca gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg 3781 ctagtgtaga tcactactag agtcacacag gaaacctact agatggtgaa tgtgaaacca 3841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 3961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 4021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 4081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 4141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 4201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 4261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 4321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 4381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 4441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 4501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 4621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 4681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 4741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 4801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac 4921 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 4981 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 5041 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 5101 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 5161 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 5221 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 5281 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 5341 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 5401 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 5461 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 5521 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 5581 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 5641 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 5701 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 5761 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5821 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5881 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt 5941 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc 6001 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc 6061 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga 6121 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat 6181 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac 6241 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc 6301 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc 6361 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat 6421 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt 6481 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac 6541 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt 6601 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt 6661 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta 6721 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt 6781 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca 6841 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc 6901 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa 6961 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 7021 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 7081 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagtcac acaggaaacc 7141 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc 7201 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg 7261 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa 7321 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg 7381 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg 7441 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc 7501 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct 7561 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc 7621 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc 7681 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt 7741 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa 7801 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag 7861 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc 7921 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat 7981 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg 8041 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc 8101 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa 8161 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 8221 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga 8281 tagtgctagt gtagatctct actagagtca cacaggaaag tactagatgt ccagattaga 8341 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 8401 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 8461 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 8521 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 8581 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 8641 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 8701 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 8761 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 8821 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 8881 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 8941 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 9001 gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 9061 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 9121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 9181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 9241 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 9301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 9361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 9421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 9481 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 9541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 9601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 9661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 9721 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 9781 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 9841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 9901 ctggctcacc ttcgggtggg cctttctgcg tttata //