LOCUS BBa_J24781 8801 bp DNA linear 14-May-2024 DEFINITION 98. 73(BBa_J24754) == 53(BBa_J24733) ACCESSION BBa_J24781 VERSION BBa_J24781.1 FEATURES Location/Qualifiers misc_feature 7..10 /label=conserved RBS 1..14 /label=BBa_B0031 CDS 24..640 /label=tetR misc_feature 641..674 /label=SsrA misc_feature 681..705 /label=Help:Barcodes CDS 21..680 /label=BBa_C0040 misc_feature 718..721 /label=conserved RBS 714..725 /label=BBa_B0034 CDS 732..1493 /label=CFP (LVA) misc_feature 1450..1487 /label=SsrA misc_feature 1488..1493 /label=2 misc_feature 800..800 /label=A misc_feature 732..1493 /label=BBa_E0022 misc_feature 732..1493 /label=BBa_E0022 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 misc_feature 1639..1657 /label=TetR 1 misc_feature 1639..1692 /label=BBa_R0040 promoter 1658..1663 /label=-35 misc_feature 1664..1682 /label=TetR 2 promoter 1681..1686 /label=-10 promoter 1639..1692 /label=BBa_R0040 RBS 1701..1713 /label=RBS-3\Weak RBS 1707..1710 /label=RBS misc_feature 1701..1713 /label=BBa_B0032 RBS 1701..1713 /label=BBa_B0032 CDS 1720..2595 /label=araC misc_feature 2596..2628 /label=LVA misc_feature 2635..2659 /label=Help:Barcodes CDS 1720..2634 /label=BBa_C0080 misc_feature 2674..2677 /label=conserved RBS 2668..2681 /label=BBa_B0031 misc_feature 3777..3815 /label=LVA CDS 2688..3815 /label=lacI-LVA misc_feature 2688..3815 /label=BBa_C0012 misc_feature 3816..3840 /label=Help:Barcodes CDS 2688..3815 /label=BBa_C0012 misc_feature 3853..3856 /label=conserved RBS 3849..3860 /label=BBa_B0034 CDS 3867..4628 /label=YFP (LVA) misc_feature 4623..4628 /label=2 misc_feature 4584..4622 /label=SsrA misc_feature 4108..4108 /label=A (Q->L) misc_feature 3867..4628 /label=BBa_E0032 misc_feature 3867..4628 /label=BBa_E0032 stem_loop 4648..4691 /label=stem_loop misc_feature 4637..4716 /label=BBa_B0010 terminator 4637..4716 /label=BBa_B0010 stem_loop 4732..4751 /label=T7 TE misc_feature 4758..4758 /label=stop polyA_site 4752..4765 /label=polya misc_feature 4725..4765 /label=BBa_B0012 terminator 4725..4765 /label=BBa_B0012 promoter 5853..5983 /label=Pbad promoter 5816..5887 /label=PC misc_feature 4774..5852 /label=AraC promoter 4774..5983 /label=BBa_I0500 misc_feature 5998..6001 /label=conserved RBS 5992..6005 /label=BBa_B0031 misc_feature 7101..7139 /label=LVA CDS 6012..7139 /label=lacI-LVA misc_feature 6012..7139 /label=BBa_C0012 misc_feature 7140..7164 /label=Help:Barcodes CDS 6012..7139 /label=BBa_C0012 RBS 7173..7185 /label=RBS-3\Weak RBS 7179..7182 /label=RBS misc_feature 7173..7185 /label=BBa_B0032 RBS 7173..7185 /label=BBa_B0032 CDS 7195..7811 /label=tetR misc_feature 7812..7845 /label=SsrA misc_feature 7852..7876 /label=Help:Barcodes CDS 7192..7851 /label=BBa_C0040 misc_feature 7889..7892 /label=conserved RBS 7885..7896 /label=BBa_B0034 CDS 7903..8664 /label=CFP (LVA) misc_feature 8621..8658 /label=SsrA misc_feature 8659..8664 /label=2 misc_feature 7971..7971 /label=A misc_feature 7903..8664 /label=BBa_E0022 misc_feature 7903..8664 /label=BBa_E0022 stem_loop 8684..8727 /label=stem_loop misc_feature 8673..8752 /label=BBa_B0010 terminator 8673..8752 /label=BBa_B0010 stem_loop 8768..8787 /label=T7 TE misc_feature 8794..8794 /label=stop polyA_site 8788..8801 /label=polya misc_feature 8761..8801 /label=BBa_B0012 terminator 8761..8801 /label=BBa_B0012 ORIGIN 1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat 61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg 721 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 781 tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg 841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 901 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg 961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 1081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 1141 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca 1201 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg 1261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 1381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1441 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 1501 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1561 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1621 tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat cagtgataga 1681 gatactgagc actactagag tcacacagga aagtactaga tggctgaagc gcaaaatgat 1741 cccctgctgc cgggatactc gtttaacgcc catctggtgg cgggtttaac gccgattgag 1801 gccaatggtt atctcgattt ttttatcgac cgaccgctgg gaatgaaagg ttatattctc 1861 aatctcacca ttcgcggtca gggggtggtg aaaaatcagg gacgagaatt tgtctgccga 1921 ccgggtgata ttttgctgtt cccgccagga gagattcatc actacggtcg tcatccggag 1981 gctcgcgaat ggtatcacca gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg 2041 cttaactggc cgtcaatatt tgccaatacg ggtttctttc gcccggatga agcgcaccag 2101 ccgcatttca gcgacctgtt tgggcaaatc attaacgccg ggcaagggga agggcgctat 2161 tcggagctgc tggcgataaa tctgcttgag caattgttac tgcggcgcat ggaagcgatt 2221 aacgagtcgc tccatccacc gatggataat cgggtacgcg aggcttgtca gtacatcagc 2281 gatcacctgg cagacagcaa ttttgatatc gccagcgtcg cacagcatgt ttgcctgtcg 2341 ccgtcgcgtc tgtcacatct tttccgccag cagttaggga ttagcgtctt aagctggcgc 2401 gaggaccaac gcatcagcca ggcgaagctg cttttgagca ctacccggat gcctatcgcc 2461 accgtcggtc gcaatgttgg ttttgacgat caactctatt tctcgcgagt atttaaaaaa 2521 tgcaccgggg ccagcccgag cgagttccgt gccggttgtg aagaaaaagt gaatgatgta 2581 gccgtcaagt tgtcagctgc aaacgacgaa aactacgctt tagtagctta ataacactga 2641 tagtgctagt gtagatcact actagagtca cacaggaaac ctactagatg gtgaatgtga 2701 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 2761 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 2821 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 2881 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 2941 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 3001 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 3061 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 3121 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 3181 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 3241 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 3301 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 3361 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 3421 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 3481 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 3541 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 3601 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 3661 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 3721 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg 3781 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc 3841 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 3901 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 3961 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 4021 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct 4081 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag 4141 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 4201 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 4261 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 4321 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 4381 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 4441 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 4501 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 4561 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag 4621 cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 4681 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 4741 tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg ctacatcatt 4801 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa 4861 tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg 4921 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct 4981 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca 5041 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta 5101 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc 5161 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc 5221 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc 5281 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg 5341 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg 5401 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac 5461 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata 5521 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg 5581 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg 5641 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg 5701 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac 5761 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa 5821 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact 5881 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc 5941 gcaactctct actgtttctc catacccgtt tttttgggct agctactaga gtcacacagg 6001 aaacctacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 6061 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 6121 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 6181 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 6241 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 6301 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 6361 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 6421 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 6481 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 6541 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 6601 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 6661 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 6721 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 6781 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 6841 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 6901 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 6961 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 7021 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 7081 cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac 7141 tctgatagtg ctagtgtaga tctctactag agtcacacag gaaagtacta gatgtccaga 7201 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa 7261 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg 7321 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac 7381 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct 7441 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca 7501 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa 7561 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt 7621 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact 7681 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag 7741 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt 7801 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 7861 tgctagtgta gatcactact agagaaagag gagaaatact agatggtgag caagggcgag 7921 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac 7981 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 8041 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 8101 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 8161 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 8221 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 8281 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 8341 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 8401 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 8461 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 8521 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 8581 gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa 8641 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca 8701 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 8761 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //