LOCUS BBa_J24788 9930 bp DNA linear 06-May-2024 DEFINITION 105. 78(BBa_J24758) == 60(BBa_J24740) ACCESSION BBa_J24788 VERSION BBa_J24788.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 misc_feature 1180..1183 /label=conserved RBS 1176..1187 /label=BBa_B0034 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1176..1878 /label=BBa_J24688 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1176..2764 /label=BBa_J24707 misc_feature 1..2764 /label=BBa_J24719 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 RBS 2835..2847 /label=RBS-3\Weak RBS 2841..2844 /label=RBS misc_feature 2835..2847 /label=BBa_B0032 RBS 2835..2847 /label=BBa_B0032 CDS 2854..3729 /label=araC misc_feature 3730..3762 /label=LVA misc_feature 3769..3793 /label=Help:Barcodes CDS 2854..3793 /label=BBa_C0080 misc_feature 2773..3793 /label=BBa_J24695 misc_feature 3806..3809 /label=conserved RBS 3802..3813 /label=BBa_B0034 misc_feature 4909..4947 /label=LVA CDS 3820..4947 /label=lacI-LVA misc_feature 3820..4947 /label=BBa_C0012 misc_feature 4948..4972 /label=Help:Barcodes CDS 3820..4947 /label=BBa_C0012 misc_feature 3802..4972 /label=BBa_J24685 misc_feature 4985..4988 /label=conserved RBS 4981..4992 /label=BBa_B0034 CDS 4999..5760 /label=YFP (LVA) misc_feature 5755..5760 /label=2 misc_feature 5716..5754 /label=SsrA misc_feature 5240..5240 /label=A (Q->L) misc_feature 4999..5760 /label=BBa_E0032 misc_feature 4999..5760 /label=BBa_E0032 stem_loop 5780..5823 /label=stem_loop misc_feature 5769..5848 /label=BBa_B0010 terminator 5769..5848 /label=BBa_B0010 stem_loop 5864..5883 /label=T7 TE misc_feature 5890..5890 /label=stop polyA_site 5884..5897 /label=polya misc_feature 5857..5897 /label=BBa_B0012 terminator 5857..5897 /label=BBa_B0012 misc_feature 4981..5897 /label=BBa_J24704 misc_feature 3802..5897 /label=BBa_J24713 misc_feature 2773..5897 /label=BBa_J24728 misc_feature 1..5897 /label=BBa_J24758 promoter 6985..7115 /label=Pbad promoter 6948..7019 /label=PC misc_feature 5906..6984 /label=AraC promoter 5906..7115 /label=BBa_I0500 misc_feature 7128..7131 /label=conserved RBS 7124..7135 /label=BBa_B0034 misc_feature 8231..8269 /label=LVA CDS 7142..8269 /label=lacI-LVA misc_feature 7142..8269 /label=BBa_C0012 misc_feature 8270..8294 /label=Help:Barcodes CDS 7142..8269 /label=BBa_C0012 misc_feature 7124..8294 /label=BBa_J24685 misc_feature 5906..8294 /label=BBa_J24699 misc_feature 8307..8310 /label=conserved RBS 8303..8314 /label=BBa_B0034 CDS 8324..8940 /label=tetR misc_feature 8941..8974 /label=SsrA misc_feature 8981..9005 /label=Help:Barcodes CDS 8321..9005 /label=BBa_C0040 misc_feature 8303..9005 /label=BBa_J24688 misc_feature 9018..9021 /label=conserved RBS 9014..9025 /label=BBa_B0034 CDS 9032..9793 /label=CFP (LVA) misc_feature 9750..9787 /label=SsrA misc_feature 9788..9793 /label=2 misc_feature 9100..9100 /label=A misc_feature 9032..9793 /label=BBa_E0022 misc_feature 9032..9793 /label=BBa_E0022 stem_loop 9813..9856 /label=stem_loop misc_feature 9802..9881 /label=BBa_B0010 terminator 9802..9881 /label=BBa_B0010 stem_loop 9897..9916 /label=T7 TE misc_feature 9923..9923 /label=stop polyA_site 9917..9930 /label=polya misc_feature 9890..9930 /label=BBa_B0012 terminator 9890..9930 /label=BBa_B0012 misc_feature 9014..9930 /label=BBa_J24703 misc_feature 8303..9930 /label=BBa_J24710 misc_feature 5906..9930 /label=BBa_J24740 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagtcacac aggaaagtac tagatggctg aagcgcaaaa tgatcccctg 2881 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat 2941 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc 3001 accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt 3061 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc 3121 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac 3181 tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat 3241 ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag 3301 ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag 3361 tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac 3421 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg 3481 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac 3541 caacgcatca gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc 3601 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc 3661 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc 3721 aagttgtcag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 3781 tagtgtagat cactactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta 3841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 3901 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3961 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 4021 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 4081 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 4141 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 4201 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 4261 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 4321 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 4381 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 4441 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 4501 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 4561 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 4621 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 4681 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 4741 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 4801 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 4921 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4981 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 5041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 5101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 5161 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 5221 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 5281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 5341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 5401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 5461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5581 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 5641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5701 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 5761 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 5821 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 5881 gcctttctgc gtttatatac tagagttatg acaacttgac ggctacatca ttcacttttt 5941 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 6001 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 6061 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 6121 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 6181 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 6241 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 6301 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 6361 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 6421 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 6481 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 6541 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 6601 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 6661 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 6721 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 6781 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 6841 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 6901 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 6961 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 7021 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 7081 ctactgtttc tccatacccg tttttttggg ctagctacta gagaaagagg agaaatacta 7141 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 7201 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 7261 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 7321 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 7381 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 7441 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 7501 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 7561 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 7621 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 7681 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 7741 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 7801 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 7861 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 7921 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 7981 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 8041 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 8101 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 8161 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 8221 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 8281 ctagtgtaga tctctactag agaaagagga gaaatactag atgtccagat tagataaaag 8341 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 8401 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 8461 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 8521 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 8581 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 8641 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 8701 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 8761 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 8821 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 8881 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 8941 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 9001 atcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 9061 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt 9121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 9181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca 9241 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 9301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 9361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 9421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa 9481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 9541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 9601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 9661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 9721 cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt 9781 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 9841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 9901 caccttcggg tgggcctttc tgcgtttata //