LOCUS       BBa_J28017              2087 bp    DNA     linear       04-May-2024
DEFINITION  Operon 3 (d)
ACCESSION   BBa_J28017
VERSION     BBa_J28017.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    211..228
                     /label=OP2
     misc_feature    271..290
                     /label=OP1
     promoter        282..287
                     /label=-35
     promoter        305..310
                     /label=-10
     promoter        166..322
                     /label=BBa_R0079
     misc_feature    335..338
                     /label=conserved
     RBS             331..342
                     /label=BBa_B0034
     misc_feature    349..351
                     /label=start
     misc_feature    1132..1137
                     /label=2
     misc_feature    1099..1131
                     /label=LVA
     CDS             349..1098
                     /label=aiiA
     misc_feature    349..1137
                     /label=BBa_C0060
     misc_feature    1138..1162
                     /label=Help:Barcodes
     CDS             349..1137
                     /label=BBa_C0060
     misc_feature    1175..1178
                     /label=conserved
     RBS             1171..1182
                     /label=BBa_B0034
     misc_feature    1945..1950
                     /label=stop
     misc_feature    1906..1944
                     /label=AAV
     misc_feature    1189..1950
                     /label=BBa_E0034
     stem_loop       1970..2013
                     /label=stem_loop
     misc_feature    1959..2038
                     /label=BBa_B0010
     terminator      1959..2038
                     /label=BBa_B0010
     stem_loop       2054..2073
                     /label=T7 TE
     misc_feature    2080..2080
                     /label=stop
     polyA_site      2074..2087
                     /label=polya
     misc_feature    2047..2087
                     /label=BBa_B0012
     terminator      2047..2087
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagaggcccc tcgctgagcg
      181 cgtcccggag ctgggggcaa cctagctgcc acctgctttt ctgctagcta ttccagcgaa
      241 aacatacaga tttccggcga aatcaaggct acctgccagt tctggcaggt ttggccgcgg
      301 gttctttttg gtacacgaaa gctactagag aaagaggaga aatactagat gacagtaaag
      361 aagctttatt tcgtcccagc aggtcgttgt atgttggatc attcgtctgt taatagtaca
      421 ttaacaccag gagaattatt agacttaccg gtttggtgtt atcttttgga gactgaagaa
      481 ggacctattt tagtagatac aggtatgcca gaaagtgcag ttaataatga aggtcttttt
      541 aacggtacat ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga tagaatcgtg
      601 aatattttaa aacgggttgg ttatgagccg gaagaccttc tttatattat tagttctcac
      661 ttgcattttg atcatgcagg aggaaatggc gcttttataa atacaccaat cattgtacag
      721 cgtgctgaat atgaggcggc gcagcatagc gaagaatatt tgaaagaatg tatattgccg
      781 aatttaaact acaaaatcat tgaaggtgat tatgaagtcg taccaggagt tcaattattg
      841 catacaccag gccatactcc agggcatcaa tcgctattaa ttgagacaga aaaatccggt
      901 cctgtattat taacgattga tgcatcgtat acgaaagaga attttgaaaa tgaagtgcca
      961 tttgcgggat ttgattcaga attagcttta tcttcaatta aacgtttaaa agaagtggtg
     1021 atgaaagaga agccgattgt tttctttgga catgatatag agcaggaaag gggatgtaaa
     1081 gtgttccctg aatatatagc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc
     1141 tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat ggtgagcaag
     1201 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1261 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1321 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1381 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
     1441 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1501 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1561 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1621 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1681 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1741 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1801 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1861 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
     1921 gacgaaaact acgctgctgc tgtttaataa tactagagcc aggcatcaaa taaaacgaaa
     1981 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2041 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//