LOCUS BBa_J28025 4849 bp DNA linear 14-May-2024 DEFINITION Intermediate System Part 2 of 2 ACCESSION BBa_J28025 VERSION BBa_J28025.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..873 /label=lasR misc_feature 874..906 /label=LVA misc_feature 636..636 /label=G misc_feature 913..937 /label=Help:Barcodes CDS 157..937 /label=BBa_C0079 stem_loop 957..1000 /label=stem_loop misc_feature 946..1025 /label=BBa_B0010 terminator 946..1025 /label=BBa_B0010 stem_loop 1041..1060 /label=T7 TE misc_feature 1067..1067 /label=stop polyA_site 1061..1074 /label=polya misc_feature 1034..1074 /label=BBa_B0012 terminator 1034..1074 /label=BBa_B0012 terminator 946..1074 /label=BBa_B0015 misc_feature 1128..1145 /label=OP2 misc_feature 1188..1207 /label=OP1 promoter 1199..1204 /label=-35 promoter 1222..1227 /label=-10 promoter 1083..1239 /label=BBa_R0079 misc_feature 1252..1255 /label=conserved RBS 1248..1259 /label=BBa_B0034 CDS 1266..1868 /label=LasI misc_feature 1869..1901 /label=LVA misc_feature 1908..1932 /label=Help:Barcodes CDS 1266..1932 /label=BBa_C0078 misc_feature 1945..1948 /label=conserved RBS 1941..1952 /label=BBa_B0034 CDS 1959..2681 /label=BBa_E0020 stem_loop 2701..2744 /label=stem_loop misc_feature 2690..2769 /label=BBa_B0010 terminator 2690..2769 /label=BBa_B0010 stem_loop 2785..2804 /label=T7 TE misc_feature 2811..2811 /label=stop polyA_site 2805..2818 /label=polya misc_feature 2778..2818 /label=BBa_B0012 terminator 2778..2818 /label=BBa_B0012 terminator 2690..2818 /label=BBa_B0015 promoter 2827..2956 /label=BBa_I13453 misc_feature 2969..2972 /label=conserved RBS 2965..2976 /label=BBa_B0034 misc_feature 2983..2984 /label=prefix misc_feature 3156..3156 /label=T misc_feature 3474..3474 /label=A CDS 2983..3732 /label=luxR misc_feature 2983..3738 /label=BBa_C0062 misc_feature 3739..3763 /label=Help:Barcodes CDS 2983..3738 /label=BBa_C0062 stem_loop 3783..3826 /label=stem_loop misc_feature 3772..3851 /label=BBa_B0010 terminator 3772..3851 /label=BBa_B0010 stem_loop 3867..3886 /label=T7 TE misc_feature 3893..3893 /label=stop polyA_site 3887..3900 /label=polya misc_feature 3860..3900 /label=BBa_B0012 terminator 3860..3900 /label=BBa_B0012 terminator 3772..3900 /label=BBa_B0015 misc_feature 3909..3928 /label=LuxR/HSL promoter 3928..3933 /label=-35 promoter 3950..3955 /label=-10 misc_feature 3961..3961 /label=start misc_feature 3909..3963 /label=BBa_R0062 promoter 3909..3963 /label=BBa_R0062 misc_feature 3976..3979 /label=conserved RBS 3972..3983 /label=BBa_B0034 CDS 3990..4712 /label=BBa_E0030 stem_loop 4732..4775 /label=stem_loop misc_feature 4721..4800 /label=BBa_B0010 terminator 4721..4800 /label=BBa_B0010 stem_loop 4816..4835 /label=T7 TE misc_feature 4842..4842 /label=stop polyA_site 4836..4849 /label=polya misc_feature 4809..4849 /label=BBa_B0012 terminator 4809..4849 /label=BBa_B0012 terminator 4721..4849 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgg ccttggttga cggttttctt 181 gagctggaac gctcaagtgg aaaattggag tggagcgcca tcctccagaa gatggcgagc 241 gaccttggat tctcgaagat cctgttcggc ctgttgccta aggacagcca ggactacgag 301 aacgccttca tcgtcggcaa ctacccggcc gcctggcgcg agcattacga ccgggctggc 361 tacgcgcggg tcgacccgac ggtcagtcac tgtacccaga gcgtactgcc gattttctgg 421 gaaccgtcca tctaccagac gcgaaagcag cacgagttct tcgaggaagc ctcggccgcc 481 ggcctggtgt atgggctgac catgccgctg catggtgctc gcggcgaact cggcgcgctg 541 agcctcagcg tggaagcgga aaaccgggcc gaggccaacc gtttcataga gtcggtcctg 601 ccgaccctgt ggatgctcaa ggactacgca ctgcaaagcg gtgccggact ggccttcgaa 661 catccggtca gcaaaccggt ggttctgacc agccgggaga aggaagtgtt gcagtggtgc 721 gccatcggca agaccagttg ggagatatcg gttatctgca actgctcgga agccaatgtg 781 aacttccata tgggaaatat tcggcggaag ttcggtgtga cctcccgccg cgtagcggcc 841 attatggccg ttaatttggg tcttattact ctcgctgcaa acgacgaaaa ctacgcttta 901 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa 961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1081 aggcccctcg ctgagcgcgt cccggagctg ggggcaacct agctgccacc tgcttttctg 1141 ctagctattc cagcgaaaac atacagattt ccggcgaaat caaggctacc tgccagttct 1201 ggcaggtttg gccgcgggtt ctttttggta cacgaaagct actagagaaa gaggagaaat 1261 actagatgat cgttcagatc ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa 1321 tgcacaaact gcgtgctcag gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg 1381 acgaaatgga aatcgacggt tacgacgctc tgtccccgta ctacatgctg atccaggaag 1441 acaccccgga agctcaggtt ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca 1501 tgctgaaaaa caccttcccg gaactgctgc acggtaaaga agctccgtgc tccccgcaca 1561 tctgggaact gtcccgtttc gctatcaact ccggtcagaa aggttccctg ggtttctccg 1621 actgcaccct ggaagctatg cgtgctctgg ctcgttactc cttgcagaac gacatccaga 1681 ccctggttac cgttaccacc gttggtgttg aaaaaatgat gatccgtgct ggtctggacg 1741 tttcccgttt cggtccgcac ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg 1801 aactgaacgc taaaacccag atcgctctgt acggtggtgt tctggttgaa cagcgtctgg 1861 ctgtttccgc tgctaacgac gaaaactacg ctctggttgc ttaataactc tgatagtgct 1921 agtgtagatc tctactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc 1981 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt 2041 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 2101 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg 2161 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 2221 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 2281 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 2341 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca 2401 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 2461 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 2521 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 2581 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 2641 ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa 2701 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 2761 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 2821 ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2881 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc 2941 gtttttttgg gctagctact agagaaagag gagaaatact agatgaaaaa cataaatgcc 3001 gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat 3061 caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt 3121 tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg 3181 aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac 3241 tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct 3301 ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt 3361 catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat 3421 atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt 3481 gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga 3541 gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata 3601 ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat 3661 acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca 3721 tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 3781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3841 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 3901 tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt atagtcgaat 3961 aaatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 4021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 4081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 4141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 4201 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 4261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 4321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 4381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 4441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 4501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 4561 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 4621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 4681 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 4741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 4801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //