LOCUS       BBa_J28030              2069 bp    DNA     linear       14-May-2024
DEFINITION  las autoinducer, P(BAD)/araC control
ACCESSION   BBa_J28030
VERSION     BBa_J28030.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..873
                     /label=lasR
     misc_feature    874..906
                     /label=LVA
     misc_feature    636..636
                     /label=G
     misc_feature    913..937
                     /label=Help:Barcodes
     CDS             157..937
                     /label=BBa_C0079
     stem_loop       957..1000
                     /label=stem_loop
     misc_feature    946..1025
                     /label=BBa_B0010
     terminator      946..1025
                     /label=BBa_B0010
     stem_loop       1041..1060
                     /label=T7 TE
     misc_feature    1067..1067
                     /label=stop
     polyA_site      1061..1074
                     /label=polya
     misc_feature    1034..1074
                     /label=BBa_B0012
     terminator      1034..1074
                     /label=BBa_B0012
     terminator      946..1074
                     /label=BBa_B0015
     misc_feature    1128..1145
                     /label=OP2
     misc_feature    1188..1207
                     /label=OP1
     promoter        1199..1204
                     /label=-35
     promoter        1222..1227
                     /label=-10
     promoter        1083..1239
                     /label=BBa_R0079
     misc_feature    1252..1255
                     /label=conserved
     RBS             1248..1259
                     /label=BBa_B0034
     CDS             1266..1868
                     /label=LasI
     misc_feature    1869..1901
                     /label=LVA
     misc_feature    1908..1932
                     /label=Help:Barcodes
     CDS             1266..1932
                     /label=BBa_C0078
     stem_loop       1952..1995
                     /label=stem_loop
     misc_feature    1941..2020
                     /label=BBa_B0010
     terminator      1941..2020
                     /label=BBa_B0010
     stem_loop       2036..2055
                     /label=T7 TE
     misc_feature    2062..2062
                     /label=stop
     polyA_site      2056..2069
                     /label=polya
     misc_feature    2029..2069
                     /label=BBa_B0012
     terminator      2029..2069
                     /label=BBa_B0012
     terminator      1941..2069
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg ccttggttga cggttttctt
      181 gagctggaac gctcaagtgg aaaattggag tggagcgcca tcctccagaa gatggcgagc
      241 gaccttggat tctcgaagat cctgttcggc ctgttgccta aggacagcca ggactacgag
      301 aacgccttca tcgtcggcaa ctacccggcc gcctggcgcg agcattacga ccgggctggc
      361 tacgcgcggg tcgacccgac ggtcagtcac tgtacccaga gcgtactgcc gattttctgg
      421 gaaccgtcca tctaccagac gcgaaagcag cacgagttct tcgaggaagc ctcggccgcc
      481 ggcctggtgt atgggctgac catgccgctg catggtgctc gcggcgaact cggcgcgctg
      541 agcctcagcg tggaagcgga aaaccgggcc gaggccaacc gtttcataga gtcggtcctg
      601 ccgaccctgt ggatgctcaa ggactacgca ctgcaaagcg gtgccggact ggccttcgaa
      661 catccggtca gcaaaccggt ggttctgacc agccgggaga aggaagtgtt gcagtggtgc
      721 gccatcggca agaccagttg ggagatatcg gttatctgca actgctcgga agccaatgtg
      781 aacttccata tgggaaatat tcggcggaag ttcggtgtga cctcccgccg cgtagcggcc
      841 attatggccg ttaatttggg tcttattact ctcgctgcaa acgacgaaaa ctacgcttta
      901 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1081 aggcccctcg ctgagcgcgt cccggagctg ggggcaacct agctgccacc tgcttttctg
     1141 ctagctattc cagcgaaaac atacagattt ccggcgaaat caaggctacc tgccagttct
     1201 ggcaggtttg gccgcgggtt ctttttggta cacgaaagct actagagaaa gaggagaaat
     1261 actagatgat cgttcagatc ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa
     1321 tgcacaaact gcgtgctcag gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg
     1381 acgaaatgga aatcgacggt tacgacgctc tgtccccgta ctacatgctg atccaggaag
     1441 acaccccgga agctcaggtt ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca
     1501 tgctgaaaaa caccttcccg gaactgctgc acggtaaaga agctccgtgc tccccgcaca
     1561 tctgggaact gtcccgtttc gctatcaact ccggtcagaa aggttccctg ggtttctccg
     1621 actgcaccct ggaagctatg cgtgctctgg ctcgttactc cttgcagaac gacatccaga
     1681 ccctggttac cgttaccacc gttggtgttg aaaaaatgat gatccgtgct ggtctggacg
     1741 tttcccgttt cggtccgcac ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg
     1801 aactgaacgc taaaacccag atcgctctgt acggtggtgt tctggttgaa cagcgtctgg
     1861 ctgtttccgc tgctaacgac gaaaactacg ctctggttgc ttaataactc tgatagtgct
     1921 agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1981 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2041 accttcgggt gggcctttct gcgtttata
//