LOCUS BBa_J29010 3667 bp DNA linear 19-May-2024 DEFINITION lac I and arabinose induable CtrI generator ACCESSION BBa_J29010 VERSION BBa_J29010.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 terminator 1180..1308 /label=BBa_B0015 misc_feature 1319..1335 /label=lac O1 promoter 1336..1341 /label=-35 misc_feature 1342..1358 /label=lac O1 promoter 1359..1364 /label=-10 misc_feature 1317..1370 /label=BBa_R0011 promoter 1317..1370 /label=BBa_R0011 misc_feature 1..1371 /label=BBa_Q04121 promoter 2459..2589 /label=Pbad promoter 2422..2493 /label=PC misc_feature 1380..2458 /label=AraC promoter 1380..2589 /label=BBa_I0500 RBS 2598..2612 /label=RBS-1\Strong RBS 2605..2609 /label=RBS misc_feature 2598..2612 /label=BBa_B0030 RBS 2598..2612 /label=BBa_B0030 misc_feature 3525..3530 /label=Double stop codon CDS 2619..3530 /label=CrtE coding sequence CDS 2619..3530 /label=BBa_J29000 stem_loop 3550..3593 /label=stem_loop misc_feature 3539..3618 /label=BBa_B0010 terminator 3539..3618 /label=BBa_B0010 stem_loop 3634..3653 /label=T7 TE misc_feature 3660..3660 /label=stop polyA_site 3654..3667 /label=polya misc_feature 3627..3667 /label=BBa_B0012 terminator 3627..3667 /label=BBa_B0012 misc_feature 1380..3667 /label=BBa_J29007 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagagt 1381 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc 1441 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca 1501 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg 1561 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga 1621 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg 1681 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt 1741 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt 1801 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac 1861 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat 1921 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg 1981 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac 2041 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga 2101 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa 2161 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag 2221 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc 2281 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg 2341 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg 2401 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac 2461 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta 2521 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt 2581 tgggctagct actagagatt aaagaggaga aatactagat gacggtctgc gcaaaaaaac 2641 acgttcatct cactcgcgat gctgcggagc agttactggc tgatattgat cgacgccttg 2701 atcagttatt gcccgtggag ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg 2761 cgctggcacc gggaaaacgt attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg 2821 gttgcgctgt cagccatgac ggattactgg atttggcctg tgcggtggaa atggtccacg 2881 cggcttcgct gatccttgac gatatgccct gcatggacga tgcgaagctg cggcgcggac 2941 gccctaccat tcattctcat tacggagagc atgtggcaat actggcggcg gttgccttgc 3001 tgagtaaagc ctttggcgta attgccgatg cagatggcct cacgccgctg gcaaaaaatc 3061 gggcggtttc tgaactgtca aacgccatcg gcatgcaagg attggttcag ggtcagttca 3121 aggatctgtc tgaaggggat aagccgcgca gcgctgaagc tattttgatg acgaatcact 3181 ttaaaaccag cacgctgttt tgtgcctcca tgcagatggc ctcgattgtt gcgaatgcct 3241 ccagcgaagc gcgtgattgc ctgcatcgtt tttcacttga tcttggtcag gcatttcaac 3301 tgctggacga tttgaccgat ggcatgaccg acaccggtaa ggatagcaat caggacgccg 3361 gtaaatcgac gctggtcaat ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac 3421 atcttcagct tgccagtgag catctctctg cggcctgcca acacgggcac gccactcaac 3481 attttattca ggcctggttt gacaaaaaac tcgctgccgt cagttaataa tactagagcc 3541 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3601 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3661 gtttata //