LOCUS       BBa_J31012              1589 bp    DNA     linear       20-May-2024
DEFINITION  Tet resistance expression cassette
ACCESSION   BBa_J31012
VERSION     BBa_J31012.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..163
                     /label=BBa_J44000
     promoter        172..301
                     /label=BBa_I13453
     misc_feature    310..335
                     /label=BBa_J44000
     RBS             344..358
                     /label=RBS-1\Strong
     RBS             351..355
                     /label=RBS
     misc_feature    344..358
                     /label=BBa_B0030
     RBS             344..358
                     /label=BBa_B0030
     CDS             365..1555
                     /label=tetA(C) forward
     primer_bind     365..388
                     /label=Fwd primer J31007
     primer_bind     1536..1555
                     /label=Rev primer J31007
     misc_feature    1553..1555
                     /label=stop
     CDS             365..1555
                     /label=BBa_J31007
     misc_feature    1564..1589
                     /label=BBa_J44000
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtta tcaaaaacca tggtttttga taatactaga gacattgatt
      181 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
      241 ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt tttgggctag
      301 ctactagagt tatcaaaaac catggttttt gataatacta gagattaaag aggagaaata
      361 ctagatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
      421 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
      481 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
      541 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
      601 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct
      661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      901 cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
      961 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     1021 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     1081 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     1141 cgccctcgct caagccttcg tcactggccc cgccaccaaa cgtttcggcg agaagcaggc
     1201 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     1261 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     1321 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     1381 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta
     1441 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     1501 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctaatacta
     1561 gagttatcaa aaaccatggt ttttgataa
//