LOCUS       BBa_J3104               1640 bp    DNA     linear       14-May-2024
DEFINITION  pBad-RBS-HixC-TetF-HixC-TT-RE
ACCESSION   BBa_J3104
VERSION     BBa_J3104.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     misc_feature    162..187
                     /label=BBa_J44000
     CDS             194..1384
                     /label=tetA(C) forward
     primer_bind     194..217
                     /label=Fwd primer J31007
     primer_bind     1365..1384
                     /label=Rev primer J31007
     misc_feature    1382..1384
                     /label=stop
     CDS             194..1384
                     /label=BBa_J31007
     misc_feature    1393..1418
                     /label=BBa_J44000
     stem_loop       1438..1481
                     /label=stem_loop
     misc_feature    1427..1506
                     /label=BBa_B0010
     terminator      1427..1506
                     /label=BBa_B0010
     stem_loop       1522..1541
                     /label=T7 TE
     misc_feature    1548..1548
                     /label=stop
     polyA_site      1542..1555
                     /label=polya
     misc_feature    1515..1555
                     /label=BBa_B0012
     terminator      1515..1555
                     /label=BBa_B0012
     misc_feature    1614..1619
                     /label=Former SpeI site
     misc_feature    1614..1614
                     /label=Mutation of SpeI site
     misc_feature    1564..1640
                     /label=RE Sequence
     misc_feature    1592..1592
                     /label=Originally a C
     misc_feature    1640..1640
                     /label=Insertion right before biobrick ends
     misc_feature    1569..1584
                     /label=Proximal Fis Binding Site
     misc_feature    1618..1632
                     /label=Distal Fis Binding Site
     misc_feature    1564..1640
                     /label=BBa_J3101
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga gttatcaaaa accatggttt
      181 ttgataatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
      241 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
      301 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
      361 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
      421 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
      481 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      541 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      601 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      661 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      721 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
      781 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
      841 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
      901 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
      961 aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac gtttcggcga
     1021 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     1081 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     1141 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     1201 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
     1261 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     1321 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     1381 ctaatactag agttatcaaa aaccatggtt tttgataata ctagagccag gcatcaaata
     1441 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1501 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1561 gagttcgggt gtcaacaatt gaccaaaata ttgatttaca gcgtaatgcg ctttctagtg
     1621 caaattgtga ccgcattttg
//