LOCUS       BBa_J3106               1608 bp    DNA     linear       28-May-2024
DEFINITION  pBad-HixC-TetB-RBS<sub>rev</sub>-HixC-TT-RE
ACCESSION   BBa_J3106
VERSION     BBa_J3106.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    139..164
                     /label=BBa_J44000
     misc_feature    1..164
                     /label=BBa_S03529
     CDS             173..1363
                     /label=tetA(C) backwards
     misc_feature    173..175
                     /label=stop
     primer_bind     173..192
                     /label=Rev primer J31006
     primer_bind     1340..1363
                     /label=Fwd primer J31006
     CDS             173..1363
                     /label=BBa_J31006
     RBS             1372..1386
                     /label=Reverse RBS
     RBS             1372..1386
                     /label=BBa_J44001
     misc_feature    173..1386
                     /label=BBa_S03532
     misc_feature    1..1386
                     /label=BBa_S03533
     stem_loop       1406..1449
                     /label=stem_loop
     misc_feature    1395..1474
                     /label=BBa_B0010
     terminator      1395..1474
                     /label=BBa_B0010
     stem_loop       1490..1509
                     /label=T7 TE
     misc_feature    1516..1516
                     /label=stop
     polyA_site      1510..1523
                     /label=polya
     misc_feature    1483..1523
                     /label=BBa_B0012
     terminator      1483..1523
                     /label=BBa_B0012
     misc_feature    1582..1587
                     /label=Former SpeI site
     misc_feature    1582..1582
                     /label=Mutation of SpeI site
     misc_feature    1532..1608
                     /label=RE Sequence
     misc_feature    1560..1560
                     /label=Originally a C
     misc_feature    1608..1608
                     /label=Insertion right before biobrick ends
     misc_feature    1537..1552
                     /label=Proximal Fis Binding Site
     misc_feature    1586..1600
                     /label=Distal Fis Binding Site
     misc_feature    1532..1608
                     /label=BBa_J3101
     misc_feature    1395..1608
                     /label=BBa_S03516
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtt atcaaaaacc atggtttttg ataatactag agttaggtcg
      181 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
      241 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
      301 cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
      361 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
      421 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
      481 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc
      541 cgaaacgttt ggtggcgggg ccagtgacga aggcttgagc gagggcgtgc aagattccga
      601 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
      661 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
      721 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
      781 tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
      841 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
      901 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
      961 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     1021 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atccacagga
     1081 cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga
     1141 ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca
     1201 tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga
     1261 tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg
     1321 tgacggtgcc gaggatgacg atgagcgcat tgttagattt cattactaga gtttctcctc
     1381 tttaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1441 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1501 gggtgggcct ttctgcgttt atatactaga gttcgggtgt caacaattga ccaaaatatt
     1561 gatttacagc gtaatgcgct ttctagtgca aattgtgacc gcattttg
//