LOCUS BBa_J32003 6371 bp DNA linear 01-Jun-2024 DEFINITION Discriminate Killing ACCESSION BBa_J32003 VERSION BBa_J32003.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1185..4175 /label=BBa_J3200 CDS 1185..4175 /label=invasin CDS 1185..4175 /label=BBa_J32000 misc_feature 4188..4191 /label=conserved RBS 4184..4195 /label=BBa_B0034 misc_feature 4202..5485 /label=J32002 CDS 4202..5485 /label=Cytosine Deaminase CDS 4202..5485 /label=BBa_J32002 misc_feature 5498..5501 /label=conserved RBS 5494..5505 /label=BBa_B0034 CDS 5512..6234 /label=BBa_E0020 stem_loop 6254..6297 /label=stem_loop misc_feature 6243..6322 /label=BBa_B0010 terminator 6243..6322 /label=BBa_B0010 stem_loop 6338..6357 /label=T7 TE misc_feature 6364..6364 /label=stop polyA_site 6358..6371 /label=polya misc_feature 6331..6371 /label=BBa_B0012 terminator 6331..6371 /label=BBa_B0012 terminator 6243..6371 /label=BBa_B0015 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgaca ggtatgcttt 1201 atttcattta aattatgatg gttttccagc caatcagtga gtttctcttg ataaggaatg 1261 cgggaatgtc tatgtatttt aataaaataa tttcatttaa tattatttca cgaatagtta 1321 tttgtatctt tttgatatgt ggaatgttca tggctggggc ttcagaaaaa tatgatgcta 1381 acgcaccgca acaggtccag ccttattctg tctcttcatc tgcatttgaa aatctccatc 1441 ctaataatga aatggagagt tcaatcaatc ccttttccgc atcggataca gaaagaaatg 1501 ctgcaataat agatcgcgcc aataaggagc aggagactga agcggtgaat aagatgataa 1561 gcaccggggc caggttagct gcatcaggca gggcatctga tgttgctcac tcaatggtgg 1621 gcgatgcggt taatcaagaa atcaaacagt ggttaaatcg attcggtacg gctcaagtta 1681 atctgaattt tgacaaaaat ttttcgctaa aagaaagctc tcttgattgg ctggctcctt 1741 ggtatgactc tgcttcattc ctctttttta gtcagttagg tattcgcaat aaagacagcc 1801 gcaacacact taaccttggc gtcgggatac gtacattgga gaacggttgg ctgtacggac 1861 ttaatacttt ttatgataat gatttgaccg gccacaacca ccgtatcggt cttggtgccg 1921 aggcctggac cgattattta cagttggctg ccaatgggta ttttcgcctc aatggatggc 1981 actcgtcgcg tgatttctcc gactataaag agcgcccagc cactgggggg gatttgcgcg 2041 cgaatgctta tttacctgca ctcccacaac tgggggggaa gttgatgtat gagcaataca 2101 ccggtgagcg tgttgcttta tttggtaaag ataatctgca acgcaaccct tatgccgtga 2161 ctgccgggat caattacacc cccgtgcctc tactcactgt cggggtagat cagcgtatgg 2221 ggaaaagcag taagcatgaa acacagtgga acctccaaat gaactatcgc ctgggcgaga 2281 gttttcagtc gcaacttagc ccttcagcgg tggcaggaac acgtctactg gcggagagcc 2341 gctataacct tgtcgatcgt aacaataata tcgtgttgga gtatcagaaa cagcaggtgg 2401 ttaaactgac attatcgcca gcaactatct ccggcctgcc gggtcaggtt tatcaggtga 2461 acgcacaagt acaaggggca tctgctgtaa gggaaattgt ctggagtgat gccgaactga 2521 ttgccgctgg cggcacatta acaccactga gtaccacaca attcaacttg gttttaccgc 2581 cttataaacg cacagcacaa gtgagtcggg taacggacga cctgacagcc aacttttatt 2641 cgcttagtgc gctcgcggtt gatcaccaag gaaaccgatc taactcattc acattgagcg 2701 tcaccgttca gcagcctcag ttgacattaa cggcggccgt cattggtgat ggcgcaccgg 2761 ctaatgggaa aactgcaatc accgttgagt tcaccgttgc tgattttgag gggaaaccct 2821 tagccgggca ggaggtggtg ataaccacca ataatggtgc gctaccgaat aaaatcacgg 2881 aaaagacaga tgcaaatggc gtcgcgcgca ttgcattaac caatacgaca gatggcgtga 2941 cggtagtcac agcagaagtg gaggggcaac ggcaaagtgt tgatacccac tttgttaagg 3001 gtactatcgc ggcggataaa tccactctgg ctgcggtacc gacatctatc atcgctgatg 3061 gtctaatggc ttcaaccatc acgttggagt tgaaggatac ctatggggac ccgcaggctg 3121 gcgcgaatgt ggcttttgac acaaccttag gcaatatggg cgttatcacg gatcacaatg 3181 acggcactta tagcgcacca ttgaccagta ccacgttggg ggtagcaaca gtaacggtga 3241 aagtggatgg ggctgcgttc agtgtgccga gtgtgacggt taatttcacg gcagatccta 3301 ttccagatgc tggccgctcc agtttcaccg tctccacacc ggatatcttg gctgatggca 3361 cgatgagttc cacattatcc tttgtccctg tcgataagaa tggccatttt atcagtggga 3421 tgcagggctt gagttttact caaaacggtg tgccggtgag tattagcccc attaccgagc 3481 agccagatag ctataccgcg acggtggttg ggaatagtgt cggtgatgtc acaatcacgc 3541 cgcaggttga taccctgata ctgagtacat tgcagaaaaa aatatcccta ttcccggtac 3601 ctacgctgac cggtattctg gttaacgggc aaaatttcgc tacggataaa gggttcccga 3661 aaacgatctt taaaaacgcc acattccagt tacagatgga taacgatgtt gctaataata 3721 ctcagtatga gtggtcgtcg tcattcacac ccaatgtatc ggttaacgat cagggtcagg 3781 tgacgattac ctaccaaacc tatagcgaag tggctgtgac ggcgaaaagt aaaaaattcc 3841 caagttattc ggtgagttat cggttctacc caaatcggtg gatatacgat ggcggcagat 3901 cgctggtatc cagtctcgag gccagcagac aatgccaagg ttcagatatg tctgcggttc 3961 ttgaatcctc acgtgcaacc aacggaacgc gtgcgcctga cgggacattg tggggcgagt 4021 gggggagctt gaccgcgtat agttctgatt ggcaatctgg tgaatattgg gtcaaaaaga 4081 ccagcacgga ttttgaaacc atgaatatgg acacaggcgc actgcaacca gggcctgcat 4141 acttggcgtt cccgctctgt gcgctgtcaa tataatacta gagaaagagg agaaatacta 4201 gatgtcgaat aacgctttac aaacaattat taacgcccgg ttaccaggcg aagaggggct 4261 gtggcagatt catctgcagg acggaaaaat cagcgccatt gatgcgcaat ccggcgtgat 4321 gcccataact gaaaacagcc tggatgccga acaaggttta gttataccgc cgtttgtgga 4381 gccacatatt cacctggaca ccacgcaaac cgccggacaa ccgaactgga atcagtccgg 4441 cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa gcgttattaa cccatgacga 4501 tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt gccaacggca ttcagcatgt 4561 gcgtacccat gtcgatgttt cggatgcaac gctaactgcg ctgaaagcaa tgctggaagt 4621 gaagcaggaa gtcgcgccgt ggattgatct gcaaatcgtc gccttccctc aggaagggat 4681 tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg ttacgcttag gggcagatgt 4741 agtgggggcg attccgcatt ttgaatttac ccgtgaatac ggcgtggagt cgctgcataa 4801 aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac gttcactgtg atgagatcga 4861 tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg gcgcaccatg aaggcatggg 4921 cgcgcgagtc accgccagcc acaccacggc aatgcactcc tataacgggg cgtatacctc 4981 acgcctgttc cgcttgctga aaatgtccgg tattaacttt gtcgccaacc cgctggtcaa 5041 tattcatctg caaggacgtt tcgatacgta tccaaaacgt cgcggcatca cgcgcgttaa 5101 agagatgctg gagtccggca ttaacgtctg ctttggtcac gatgatgtct tcgatccgtg 5161 gtatccgctg ggaacggcga atatgctgca agtgctgcat atggggctgc atgtttgcca 5221 gttgatgggc tacgggcaga ttaacgatgg cctgaattta atcacccacc acagcgcaag 5281 gacgttgaat ttgcaggatt acggcattgc cgccggaaac agcgccaacc tgattatcct 5341 gccggctgaa aatgggtttg atgcgctgcg ccgtcaggtt ccggtacgtt attcggtacg 5401 tggcggcaag gtgattgcca gcacacaacc ggcacaaacc accgtatatc tggagcagcc 5461 agaagccatc gattacaaac gttgatacta gagaaagagg agaaatacta gatggtgagc 5521 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg 5581 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 5641 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 5701 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 5761 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 5821 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 5881 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 5941 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 6001 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 6061 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 6121 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 6181 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 6241 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6301 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6361 ctgcgtttat a //