LOCUS BBa_J32005 4028 bp DNA linear 11-May-2024 DEFINITION Regulated Suicide ACCESSION BBa_J32005 VERSION BBa_J32005.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 660..691 /label=LVA CDS 81..659 /label=luxI misc_feature 81..698 /label=BBa_C0061 misc_feature 699..723 /label=Help:Barcodes CDS 81..698 /label=BBa_C0061 stem_loop 743..786 /label=stem_loop misc_feature 732..811 /label=BBa_B0010 terminator 732..811 /label=BBa_B0010 stem_loop 827..846 /label=T7 TE misc_feature 853..853 /label=stop polyA_site 847..860 /label=polya misc_feature 820..860 /label=BBa_B0012 terminator 820..860 /label=BBa_B0012 terminator 732..860 /label=BBa_B0015 misc_feature 869..888 /label=LuxR/HSL promoter 983..990 /label=-10 promoter 957..962 /label=-35 misc_feature 996..996 /label=start misc_feature 998..1017 /label=Putative LuxR/HSL misc_feature 869..1019 /label=BBa_R0063 promoter 869..1019 /label=BBa_R0063 misc_feature 1032..1035 /label=conserved RBS 1028..1039 /label=BBa_B0034 misc_feature 1046..1047 /label=prefix misc_feature 1219..1219 /label=T misc_feature 1537..1537 /label=A CDS 1046..1795 /label=luxR misc_feature 1046..1801 /label=BBa_C0062 misc_feature 1802..1826 /label=Help:Barcodes CDS 1046..1801 /label=BBa_C0062 stem_loop 1846..1889 /label=stem_loop misc_feature 1835..1914 /label=BBa_B0010 terminator 1835..1914 /label=BBa_B0010 stem_loop 1930..1949 /label=T7 TE misc_feature 1956..1956 /label=stop polyA_site 1950..1963 /label=polya misc_feature 1923..1963 /label=BBa_B0012 terminator 1923..1963 /label=BBa_B0012 misc_feature 1972..1991 /label=LuxR/HSL promoter 1991..1996 /label=-35 promoter 2013..2018 /label=-10 misc_feature 2024..2024 /label=start misc_feature 1972..2026 /label=BBa_R0062 promoter 1972..2026 /label=BBa_R0062 misc_feature 869..2026 /label=BBa_F2621 misc_feature 2039..2042 /label=conserved RBS 2035..2046 /label=BBa_B0034 misc_feature 2053..2271 /label=J32004 CDS 2053..2271 /label=BBa_J32004 stem_loop 2291..2334 /label=stem_loop misc_feature 2280..2359 /label=BBa_B0010 terminator 2280..2359 /label=BBa_B0010 stem_loop 2375..2394 /label=T7 TE misc_feature 2401..2401 /label=stop polyA_site 2395..2408 /label=polya misc_feature 2368..2408 /label=BBa_B0012 terminator 2368..2408 /label=BBa_B0012 terminator 2280..2408 /label=BBa_B0015 misc_feature 2419..2435 /label=lac O1 promoter 2436..2441 /label=-35 misc_feature 2442..2458 /label=lac O1 promoter 2459..2464 /label=-10 misc_feature 2417..2470 /label=BBa_R0011 promoter 2417..2470 /label=BBa_R0011 misc_feature 2484..2487 /label=conserved RBS 2480..2491 /label=BBa_B0034 CDS 2498..3151 /label=BBa_J32026 misc_feature 3164..3167 /label=conserved RBS 3160..3171 /label=BBa_B0034 CDS 3178..3888 /label=mCherry misc_feature 3529..3529 /label=C->T (removing PstI site) misc_feature 3178..3891 /label=BBa_J06504 stem_loop 3911..3954 /label=stem_loop misc_feature 3900..3979 /label=BBa_B0010 terminator 3900..3979 /label=BBa_B0010 stem_loop 3995..4014 /label=T7 TE misc_feature 4021..4021 /label=stop polyA_site 4015..4028 /label=polya misc_feature 3988..4028 /label=BBa_B0012 terminator 3988..4028 /label=BBa_B0012 terminator 3900..4028 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc 121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg 181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag 241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta 301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc 361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga 421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg 481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa 541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata 601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg 661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 721 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 781 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 841 tgggcctttc tgcgtttata tactagagac ctgtacgatc ctacaggtgc ttatgttaag 901 taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag aatcaaatgt 961 caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac aataggtaat 1021 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat 1081 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat 1141 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt 1201 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga 1261 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat 1321 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga 1381 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg 1441 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt 1501 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat 1561 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc 1621 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg 1681 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca 1741 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata 1801 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 1861 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 1921 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg 1981 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact agagaaagag 2041 gagaaatact agatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg 2101 ctcaaggcat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc 2161 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc 2221 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg atactagagc 2281 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2341 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2401 cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga 2461 tactgagcac atactagaga aagaggagaa atactagatg accatgatta cgccaagcta 2521 tttaggtgac actatagaat actcaagcta tgcatcaagc ttggtaccga gctcggatcc 2581 gctagtaacg gccgccagtg tgctggagtt ctgtagatat ccatcacact ggagaccgct 2641 cgagcatgca tccagagggc ccaattcgcc ctatagtgag tcgtattaca attcactggc 2701 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 2761 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 2821 ccaacagttg cgcagcctat acgtacggca gtttaaggtt tacacctata aaagagagag 2881 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgccgg ggcgacggat 2941 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc 3001 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc 3061 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa 3121 cgccattaac ctgatgttct ggggaatata atactagaga aagaggagaa atactagatg 3181 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 3241 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 3301 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 3361 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 3421 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 3481 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc 3541 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 3601 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 3661 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 3721 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac 3781 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 3841 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaata atactagagc 3901 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3961 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 4021 cgtttata //