LOCUS BBa_J32013 4397 bp DNA linear 10-May-2024 DEFINITION Predator I ACCESSION BBa_J32013 VERSION BBa_J32013.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 misc_feature 874..877 /label=conserved RBS 870..881 /label=BBa_B0034 CDS 888..1550 /label=CinI misc_feature 1551..1583 /label=LVA misc_feature 1590..1614 /label=Help:Barcodes CDS 888..1614 /label=BBa_C0076 stem_loop 1634..1677 /label=stem_loop misc_feature 1623..1702 /label=BBa_B0010 terminator 1623..1702 /label=BBa_B0010 stem_loop 1718..1737 /label=T7 TE misc_feature 1744..1744 /label=stop polyA_site 1738..1751 /label=polya misc_feature 1711..1751 /label=BBa_B0012 terminator 1711..1751 /label=BBa_B0012 terminator 1623..1751 /label=BBa_B0015 misc_feature 1760..1779 /label=LuxR/HSL promoter 1779..1784 /label=-35 promoter 1801..1806 /label=-10 misc_feature 1812..1812 /label=start misc_feature 1760..1814 /label=BBa_R0062 promoter 1760..1814 /label=BBa_R0062 misc_feature 1827..1830 /label=conserved RBS 1823..1834 /label=BBa_B0034 CDS 1841..2691 /label=AmpR CDS 1841..2691 /label=BBa_J32020 stem_loop 2711..2754 /label=stem_loop misc_feature 2700..2779 /label=BBa_B0010 terminator 2700..2779 /label=BBa_B0010 stem_loop 2795..2814 /label=T7 TE misc_feature 2821..2821 /label=stop polyA_site 2815..2828 /label=polya misc_feature 2788..2828 /label=BBa_B0012 terminator 2788..2828 /label=BBa_B0012 terminator 2700..2828 /label=BBa_B0015 misc_feature 2839..2855 /label=lac O1 promoter 2856..2861 /label=-35 misc_feature 2862..2878 /label=lac O1 promoter 2879..2884 /label=-10 misc_feature 2837..2890 /label=BBa_R0011 promoter 2837..2890 /label=BBa_R0011 misc_feature 2904..2907 /label=conserved RBS 2900..2911 /label=BBa_B0034 CDS 2918..3520 /label=BBa_J32023 misc_feature 3533..3536 /label=conserved RBS 3529..3540 /label=BBa_B0034 CDS 3547..4257 /label=mCherry misc_feature 3898..3898 /label=C->T (removing PstI site) misc_feature 3547..4260 /label=BBa_J06504 stem_loop 4280..4323 /label=stem_loop misc_feature 4269..4348 /label=BBa_B0010 terminator 4269..4348 /label=BBa_B0010 stem_loop 4364..4383 /label=T7 TE misc_feature 4390..4390 /label=stop polyA_site 4384..4397 /label=polya misc_feature 4357..4397 /label=BBa_B0012 terminator 4357..4397 /label=BBa_B0012 terminator 4269..4397 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg ttcgttatca 901 tccaggctca cgaataccag aaatacgctg ctgttctgga ccagatgttc cgtctgcgta 961 aaaaagtttt cgctgacacc ctgtgctggg acgttccggt tatcggtccg tacgaacgtg 1021 actcctacga ctccctggct ccggcttacc tggtttggtg caacgactcc cgtacccgtc 1081 tgtacggtgg tatgcgtctg atgccgacca ccggtccgac cctgctgtac gacgttttcc 1141 gtgaaacctt cccggacgct gctgacctga tcgctccggg tatctgggaa ggtacccgta 1201 tgtgcatcga cgaagaagct atcgctaaag acttcccgga aatcgacgct ggtcgtgctt 1261 tctccatgat gctgctggct ctgtgcgaat gcgctctgga ccacggtatc cacaccatga 1321 tctccaacta cgaaccgtac ctgaaacgtg tttacaaacg tgctggtgct gaagttgaag 1381 aactgggtcg tgctgacggt tacggtaaat acccggtttg ctgcggtgct ttcgaagttt 1441 ccgaccgtgt tctgcgtaaa atgcgtgctg ctctgggtct gaccctgccg ctgtacgttc 1501 gtcacgttcc ggctcgttcc gttgttaccc agttcctgga aatggctgct gctgctaacg 1561 acgaaaacta cgctctggtt gcttaataac cctgatagtg ctagtgtaga tccctactag 1621 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1681 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1741 ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt 1801 tatagtcgaa taaatactag agaaagagga gaaatactag atgagtattc aacatttccg 1861 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 1921 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 1981 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 2041 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 2101 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 2161 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 2221 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 2281 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 2341 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 2401 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 2461 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 2521 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 2581 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 2641 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta atactagagc 2701 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2761 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2821 cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga 2881 tactgagcac atactagaga aagaggagaa atactagatg cttttatata aaatgtgtga 2941 caatcaaaat tatggggtta cttacatgaa gtttttattg gcattttcgc ttttaatacc 3001 atccgtggtt tttgcaagta gtgcaggtgt tatgacagga gcaaaattca cgcagatcca 3061 gtttggtatg acacgtcagc aggtcctcga catagcaggt gctgagaact gtgagactgg 3121 tggatcgttc ggtgacagca tccattgtcg tggacatgca gcaggagact attatgcata 3181 cgcaaccttc ggcttcacca gcgcagctgc agacgcaaag gtggattcga aaagccagga 3241 aaaactgctt gcaccaagcg caccaactct tactcttgct aagttcaacc aagtcactgt 3301 tggtatgact agagcacaag tacttgctac cgtcggacag ggttcttgta ccacttggag 3361 tgagtactat ccagcatatc catcgacggc aggagtgact ctcagcctgt cctgcttcga 3421 tgtggacggt tactcgtcga cggggttcta ccgaggctcg gcgcacctct ggttcacgga 3481 cggggtgctt cagggcaagc ggcagtggga ccttgtataa tactagagaa agaggagaaa 3541 tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 3601 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 3661 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 3721 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 3781 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 3841 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg 3901 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 3961 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 4021 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 4081 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 4141 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 4201 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaataa 4261 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 4321 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 4381 gcctttctgc gtttata //