LOCUS BBa_J32024 3577 bp DNA linear 13-May-2024 DEFINITION Predator II ACCESSION BBa_J32024 VERSION BBa_J32024.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 misc_feature 874..877 /label=conserved RBS 870..881 /label=BBa_B0034 CDS 888..1550 /label=CinI misc_feature 1551..1583 /label=LVA misc_feature 1590..1614 /label=Help:Barcodes CDS 888..1614 /label=BBa_C0076 stem_loop 1634..1677 /label=stem_loop misc_feature 1623..1702 /label=BBa_B0010 terminator 1623..1702 /label=BBa_B0010 stem_loop 1718..1737 /label=T7 TE misc_feature 1744..1744 /label=stop polyA_site 1738..1751 /label=polya misc_feature 1711..1751 /label=BBa_B0012 terminator 1711..1751 /label=BBa_B0012 terminator 1623..1751 /label=BBa_B0015 misc_feature 1760..1779 /label=LuxR/HSL promoter 1779..1784 /label=-35 promoter 1801..1806 /label=-10 misc_feature 1812..1812 /label=start misc_feature 1760..1814 /label=BBa_R0062 promoter 1760..1814 /label=BBa_R0062 misc_feature 1827..1830 /label=conserved RBS 1823..1834 /label=BBa_B0034 CDS 1841..2500 /label=BBa_J32021 stem_loop 2520..2563 /label=stem_loop misc_feature 2509..2588 /label=BBa_B0010 terminator 2509..2588 /label=BBa_B0010 stem_loop 2604..2623 /label=T7 TE misc_feature 2630..2630 /label=stop polyA_site 2624..2637 /label=polya misc_feature 2597..2637 /label=BBa_B0012 terminator 2597..2637 /label=BBa_B0012 terminator 2509..2637 /label=BBa_B0015 misc_feature 2648..2664 /label=lac O1 promoter 2665..2670 /label=-35 misc_feature 2671..2687 /label=lac O1 promoter 2688..2693 /label=-10 misc_feature 2646..2699 /label=BBa_R0011 promoter 2646..2699 /label=BBa_R0011 misc_feature 2713..2716 /label=conserved RBS 2709..2720 /label=BBa_B0034 CDS 2727..3437 /label=mCherry misc_feature 3078..3078 /label=C->T (removing PstI site) misc_feature 2727..3440 /label=BBa_J06504 stem_loop 3460..3503 /label=stem_loop misc_feature 3449..3528 /label=BBa_B0010 terminator 3449..3528 /label=BBa_B0010 stem_loop 3544..3563 /label=T7 TE misc_feature 3570..3570 /label=stop polyA_site 3564..3577 /label=polya misc_feature 3537..3577 /label=BBa_B0012 terminator 3537..3577 /label=BBa_B0012 terminator 3449..3577 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg ttcgttatca 901 tccaggctca cgaataccag aaatacgctg ctgttctgga ccagatgttc cgtctgcgta 961 aaaaagtttt cgctgacacc ctgtgctggg acgttccggt tatcggtccg tacgaacgtg 1021 actcctacga ctccctggct ccggcttacc tggtttggtg caacgactcc cgtacccgtc 1081 tgtacggtgg tatgcgtctg atgccgacca ccggtccgac cctgctgtac gacgttttcc 1141 gtgaaacctt cccggacgct gctgacctga tcgctccggg tatctgggaa ggtacccgta 1201 tgtgcatcga cgaagaagct atcgctaaag acttcccgga aatcgacgct ggtcgtgctt 1261 tctccatgat gctgctggct ctgtgcgaat gcgctctgga ccacggtatc cacaccatga 1321 tctccaacta cgaaccgtac ctgaaacgtg tttacaaacg tgctggtgct gaagttgaag 1381 aactgggtcg tgctgacggt tacggtaaat acccggtttg ctgcggtgct ttcgaagttt 1441 ccgaccgtgt tctgcgtaaa atgcgtgctg ctctgggtct gaccctgccg ctgtacgttc 1501 gtcacgttcc ggctcgttcc gttgttaccc agttcctgga aatggctgct gctgctaacg 1561 acgaaaacta cgctctggtt gcttaataac cctgatagtg ctagtgtaga tccctactag 1621 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1681 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1741 ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt 1801 tatagtcgaa taaatactag agaaagagga gaaatactag atggagaaaa aaatcactgg 1861 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 1921 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 1981 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 2041 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag 2101 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 2161 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 2221 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 2281 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 2341 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 2401 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga 2461 attacaacag tactgcgatg agtggcaggg cggggcgtaa tactagagcc aggcatcaaa 2521 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2581 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2641 tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca 2701 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggaggat aacatggcca 2761 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 2821 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 2881 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 2941 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 3001 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 3061 tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca 3121 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 3181 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 3241 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 3301 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 3361 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 3421 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3481 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3541 actggctcac cttcgggtgg gcctttctgc gtttata //