LOCUS BBa_J32025 3816 bp DNA linear 10-May-2024 DEFINITION Predator III ACCESSION BBa_J32025 VERSION BBa_J32025.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 misc_feature 874..877 /label=conserved RBS 870..881 /label=BBa_B0034 CDS 888..1550 /label=CinI misc_feature 1551..1583 /label=LVA misc_feature 1590..1614 /label=Help:Barcodes CDS 888..1614 /label=BBa_C0076 stem_loop 1634..1677 /label=stem_loop misc_feature 1623..1702 /label=BBa_B0010 terminator 1623..1702 /label=BBa_B0010 stem_loop 1718..1737 /label=T7 TE misc_feature 1744..1744 /label=stop polyA_site 1738..1751 /label=polya misc_feature 1711..1751 /label=BBa_B0012 terminator 1711..1751 /label=BBa_B0012 terminator 1623..1751 /label=BBa_B0015 misc_feature 1760..1779 /label=LuxR/HSL promoter 1779..1784 /label=-35 promoter 1801..1806 /label=-10 misc_feature 1812..1812 /label=start misc_feature 1760..1814 /label=BBa_R0062 promoter 1760..1814 /label=BBa_R0062 misc_feature 1827..1830 /label=conserved RBS 1823..1834 /label=BBa_B0034 misc_feature 1841..2059 /label=J32004 CDS 1841..2059 /label=BBa_J32004 stem_loop 2079..2122 /label=stem_loop misc_feature 2068..2147 /label=BBa_B0010 terminator 2068..2147 /label=BBa_B0010 stem_loop 2163..2182 /label=T7 TE misc_feature 2189..2189 /label=stop polyA_site 2183..2196 /label=polya misc_feature 2156..2196 /label=BBa_B0012 terminator 2156..2196 /label=BBa_B0012 terminator 2068..2196 /label=BBa_B0015 misc_feature 2207..2223 /label=lac O1 promoter 2224..2229 /label=-35 misc_feature 2230..2246 /label=lac O1 promoter 2247..2252 /label=-10 misc_feature 2205..2258 /label=BBa_R0011 promoter 2205..2258 /label=BBa_R0011 misc_feature 2272..2275 /label=conserved RBS 2268..2279 /label=BBa_B0034 CDS 2286..2939 /label=BBa_J32026 misc_feature 2952..2955 /label=conserved RBS 2948..2959 /label=BBa_B0034 CDS 2966..3676 /label=mCherry misc_feature 3317..3317 /label=C->T (removing PstI site) misc_feature 2966..3679 /label=BBa_J06504 stem_loop 3699..3742 /label=stem_loop misc_feature 3688..3767 /label=BBa_B0010 terminator 3688..3767 /label=BBa_B0010 stem_loop 3783..3802 /label=T7 TE misc_feature 3809..3809 /label=stop polyA_site 3803..3816 /label=polya misc_feature 3776..3816 /label=BBa_B0012 terminator 3776..3816 /label=BBa_B0012 terminator 3688..3816 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg ttcgttatca 901 tccaggctca cgaataccag aaatacgctg ctgttctgga ccagatgttc cgtctgcgta 961 aaaaagtttt cgctgacacc ctgtgctggg acgttccggt tatcggtccg tacgaacgtg 1021 actcctacga ctccctggct ccggcttacc tggtttggtg caacgactcc cgtacccgtc 1081 tgtacggtgg tatgcgtctg atgccgacca ccggtccgac cctgctgtac gacgttttcc 1141 gtgaaacctt cccggacgct gctgacctga tcgctccggg tatctgggaa ggtacccgta 1201 tgtgcatcga cgaagaagct atcgctaaag acttcccgga aatcgacgct ggtcgtgctt 1261 tctccatgat gctgctggct ctgtgcgaat gcgctctgga ccacggtatc cacaccatga 1321 tctccaacta cgaaccgtac ctgaaacgtg tttacaaacg tgctggtgct gaagttgaag 1381 aactgggtcg tgctgacggt tacggtaaat acccggtttg ctgcggtgct ttcgaagttt 1441 ccgaccgtgt tctgcgtaaa atgcgtgctg ctctgggtct gaccctgccg ctgtacgttc 1501 gtcacgttcc ggctcgttcc gttgttaccc agttcctgga aatggctgct gctgctaacg 1561 acgaaaacta cgctctggtt gcttaataac cctgatagtg ctagtgtaga tccctactag 1621 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1681 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1741 ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt 1801 tatagtcgaa taaatactag agaaagagga gaaatactag atgaagcagc gtattacagt 1861 gacagttgac agcgacagct atcagttgct caaggcatat gatgtcaata tctccggtct 1921 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 1981 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 2041 cgagaacagg gactggtgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2101 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2161 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa 2221 caattgacat tgtgagcgga taacaagata ctgagcacat actagagaaa gaggagaaat 2281 actagatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg 2341 catcaagctt ggtaccgagc tcggatccgc tagtaacggc cgccagtgtg ctggagttct 2401 gtagatatcc atcacactgg agaccgctcg agcatgcatc cagagggccc aattcgccct 2461 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt 2641 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg 2701 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt 2761 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca 2821 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc 2881 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataat 2941 actagagaaa gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat 3001 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 3061 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 3121 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 3181 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 3241 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 3301 gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 3361 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 3421 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 3481 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 3541 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 3601 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 3661 cgagctgtac aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3781 ctggctcacc ttcgggtggg cctttctgcg tttata //