LOCUS       BBa_J329050             1012 bp    DNA     linear       01-Jun-2024
DEFINITION  Strong AiiA Expression Plasmid
ACCESSION   BBa_J329050
VERSION     BBa_J329050.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     misc_feature    62..64
                     /label=start
     misc_feature    845..850
                     /label=2
     misc_feature    812..844
                     /label=LVA
     CDS             62..811
                     /label=aiiA
     misc_feature    62..850
                     /label=BBa_C0060
     misc_feature    851..875
                     /label=Help:Barcodes
     CDS             62..850
                     /label=BBa_C0060
     stem_loop       895..938
                     /label=stem_loop
     misc_feature    884..963
                     /label=BBa_B0010
     terminator      884..963
                     /label=BBa_B0010
     stem_loop       979..998
                     /label=T7 TE
     misc_feature    1005..1005
                     /label=stop
     polyA_site      999..1012
                     /label=polya
     misc_feature    972..1012
                     /label=BBa_B0012
     terminator      972..1012
                     /label=BBa_B0012
     terminator      884..1012
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc
      121 tgttaatagt acattaacac caggagaatt attagactta ccggtttggt gttatctttt
      181 ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg cagttaataa
      241 tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa tgactgaaga
      301 agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc ttctttatat
      361 tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta taaatacacc
      421 aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat atttgaaaga
      481 atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag tcgtaccagg
      541 agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat taattgagac
      601 agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag agaattttga
      661 aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa ttaaacgttt
      721 aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata tagagcagga
      781 aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact acgctttagt
      841 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
      901 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      961 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//