BBa_J331011
1
BBa_J331011
Water Purification from Lead
2016-12-03T12:00:00Z
2016-12-03T03:34:21Z
Anderson promoter comes from part BBa_J23119. The RBS comes from part BBa_0030. CBP4 is naturally found in Nicotina tabacum, but it also comes from part BBa_J331007. Terminator comes from part BBa_B0014. T7 promoter comes from part BBa_I719005. GST is naturally found in S. japonicum, but it also comes from part BBa_T2025. CRS5 is a metallothionein found in S. serevisiae, but also comes from part BBa_ J331009. The linking sequence that will link CRS5 and GST comes from part BBa_J331010.
This main purpose of this composite is to purify water from lead, so that it is cleaner for its use. This gene can be inserted into E.coli so that the bacteria can be placed in a tank filled with contaminated water and purify the water from lead.
false
false
_1979_
24251
24251
9
false
None
false
Gilles Yvan Buck
component2531703
1
BBa_B0030
component2531708
1
BBa_J331009
component2531690
1
BBa_J331007
component2531701
1
BBa_I719005
component2531688
1
BBa_B0030
component2531692
1
BBa_B0030
component2531686
1
BBa_J23119
component2531706
1
BBa_T2025
component2531700
1
BBa_B0014
component2531707
1
BBa_J331010
component2531710
1
BBa_B0030
component2531718
1
BBa_B0014
annotation2531718
1
BBa_B0014
range2531718
1
1710
1804
annotation2531710
1
BBa_B0030
range2531710
1
1687
1701
annotation2531703
1
BBa_B0030
range2531703
1
743
757
annotation2531706
1
BBa_T2025
range2531706
1
766
1422
annotation2531708
1
BBa_J331009
range2531708
1
1469
1678
annotation2531686
1
BBa_J23119
range2531686
1
1
35
annotation2531688
1
BBa_B0030
range2531688
1
44
58
annotation2531707
1
BBa_J331010
range2531707
1
1431
1462
annotation2531690
1
BBa_J331007
range2531690
1
65
577
annotation2531700
1
BBa_B0014
range2531700
1
609
703
annotation2531701
1
BBa_I719005
range2531701
1
712
734
annotation2531692
1
BBa_B0030
range2531692
1
586
600
BBa_T2025
1
BBa_T2025
Glutathione S-transferase internal domain
2009-09-16T11:00:00Z
2015-05-08T01:14:52Z
This is a synthetic sequence.
This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
false
false
_41_
0
126
162
Not in stock
false
Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
false
Reshma Shetty
annotation2021346
1
GST
range2021346
1
1
657
BBa_J331007
1
BBa_J331007
CBP4
2016-12-03T12:00:00Z
2016-12-03T10:23:32Z
It comes from a genomic sequence.
http://www.yeastgenome.org/locus/S000003406/sequence
Mitochondrial protein required for gathering of cytochrome bc1 complex; interfaces with the Cbp3p-Cbp6p complex and recently integrated cytochrome b (Cobp) to advance get together of Cobp into the cytochrome bc1 complex. It was used by the 2014 Cornell Project as a part to remove lead from water.
false
false
_1979_
24251
24251
9
false
None
false
Gilles Yvan Buck
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_I719005
1
pT7
T7 Promoter
2007-10-23T11:00:00Z
2015-08-31T04:07:53Z
---
Released HQ 2013
Just a T7 Promoter
false
false
_128_
0
2097
9
In stock
true
None
true
Imperial 2007
BBa_J331010
1
BBa_J331010
Linking Sequence for Metallothionein (CRS5) and Glutathione S-Transferase (GST)
2016-12-03T12:00:00Z
2016-12-03T02:29:14Z
It comes from a genomic sequence.
This is a linking sequence used to link two coding regions (CRS5 and GST) without any RBS. These two are used as part of the process of removing lead (Pb) from water.
false
false
_1979_
24251
24251
9
false
None
false
Gilles Yvan Buck
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_J331009
1
BBa_J331009
CSR5 (Metallothionein)
2016-12-03T12:00:00Z
2016-12-03T11:21:39Z
Metallothionein. Genomic Sequence
This coding region codes for the removal of heavy metals and the purification of substances. It can be used for the removal of lead, mercury, nickel, and others when combined with other biobricks to create a composite part. It was used by Cornell in 2014 to purify water from heavy metals.
false
false
_1979_
24251
24251
9
false
None
false
Gilles Yvan Buck
BBa_T2025_sequence
1
tccccgattctgggttattggaaaatcaagggtcttgtccagccgacccgccttctcctggagtatcttgaggaaaaatatgaggaacacctttacgaacgtgacgagggcgataaatggcgtaacaaaaagttcgagcttggcctcgaatttccgaatcttccgtactacattgacggcgacgtcaaactgacccagagcatggcgattatccgttacatcgcggataaacataacatgctcggcggttgtccgaaagagcgcgccgaaatttccatgcttgaaggtgccgtcctggatattcgttacggtgtgtcccgtattgcctacagcaaagactttgaaacccttaaagtggattttctctcgaaacttccggaaatgcttaaaatgttcgaagatcgtctgtgtcacaagacctacctcaacggcgaccatgtgacccacccggacttcatgctttatgacgcgcttgacgtggtgctctacatggacccgatgtgcctggacgcgttcccgaaacttgtgtgctttaaaaagcgcattgaagcgatcccgcagatcgacaagtaccttaagtccagcaagtatatcgcgtggccgcttcagggttggcaagccaccttcggtggcggtgatcacccgccgaaatccgac
BBa_I719005_sequence
1
taatacgactcactatagggaga
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_J331009_sequence
1
atgactgtaaaaatatgtgactgttaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J331007_sequence
1
atgcagtgcgcgataactcctagagaggcagtaatagcaaaacaaagacagtataagcattatttgggtatggaaagaccattgtgggtacgttggttgaaagtctatgcaattggtggcgctattattggaagcgggtttctgcttttcaagtacacaacgccaacagatcaacagttaatcagccagctttccccagaattacgtttacaatatgagagggagaaaaaactacgtcagtcggagcagcaggctttgatgaaaattgttaaggaaacttctcaaagtgacgatccaatttggaagacgggcccccttcaatcaccttgggaaaggaacggtgataacgttcaaagcagagatcactttgctaaggtgagggcggaggaggtgcaaaaagaagagttggctaggatcagaaatgaactgtctcagctaagatctgaaacggaggagaaaacaaaggaaatagtccaggataagcaggttaaaagctggtggcgcttctggtag
BBa_J331011_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgcagtgcgcgataactcctagagaggcagtaatagcaaaacaaagacagtataagcattatttgggtatggaaagaccattgtgggtacgttggttgaaagtctatgcaattggtggcgctattattggaagcgggtttctgcttttcaagtacacaacgccaacagatcaacagttaatcagccagctttccccagaattacgtttacaatatgagagggagaaaaaactacgtcagtcggagcagcaggctttgatgaaaattgttaaggaaacttctcaaagtgacgatccaatttggaagacgggcccccttcaatcaccttgggaaaggaacggtgataacgttcaaagcagagatcactttgctaaggtgagggcggaggaggtgcaaaaagaagagttggctaggatcagaaatgaactgtctcagctaagatctgaaacggaggagaaaacaaaggaaatagtccaggataagcaggttaaaagctggtggcgcttctggtagtactagagattaaagaggagaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaatacgactcactatagggagatactagagattaaagaggagaaatactagagtccccgattctgggttattggaaaatcaagggtcttgtccagccgacccgccttctcctggagtatcttgaggaaaaatatgaggaacacctttacgaacgtgacgagggcgataaatggcgtaacaaaaagttcgagcttggcctcgaatttccgaatcttccgtactacattgacggcgacgtcaaactgacccagagcatggcgattatccgttacatcgcggataaacataacatgctcggcggttgtccgaaagagcgcgccgaaatttccatgcttgaaggtgccgtcctggatattcgttacggtgtgtcccgtattgcctacagcaaagactttgaaacccttaaagtggattttctctcgaaacttccggaaatgcttaaaatgttcgaagatcgtctgtgtcacaagacctacctcaacggcgaccatgtgacccacccggacttcatgctttatgacgcgcttgacgtggtgctctacatggacccgatgtgcctggacgcgttcccgaaacttgtgtgctttaaaaagcgcattgaagcgatcccgcagatcgacaagtaccttaagtccagcaagtatatcgcgtggccgcttcagggttggcaagccaccttcggtggcggtgatcacccgccgaaatccgactactagagatcggatctggttccgcgtggatccattcatatactagatgactgtaaaaatatgtgactgttaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagtactagagattaaagaggagaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_J331010_sequence
1
atcggatctggttccgcgtggatccattcata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z