BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
BBa_B0025
1
BBa_B0025
double terminator (B0015), reversed
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
true
_1_
0
24
7
In stock
false
true
Caitlin Conboy
annotation369703
1
B0010
range369703
1
50
129
annotation369702
1
B0012
range369702
1
1
41
BBa_J37037
1
BBa_J37037
This device is to test the Pops Blocker part
2006-09-12T11:00:00Z
2015-08-31T04:08:48Z
registry
This device is to test the Pops Blocker part J37027
false
false
_66_
0
1068
66
Not in stock
false
none
false
John Chattaway
component1901672
1
BBa_R0040
component1901691
1
BBa_B0025
component1901688
1
BBa_J37027
component1901677
1
BBa_B0030
annotation1901691
1
BBa_B0025
range1901691
1
1221
1349
annotation1901677
1
BBa_B0030
range1901677
1
63
77
annotation1901672
1
BBa_R0040
range1901672
1
1
54
annotation1901688
1
BBa_J37027
range1901688
1
86
1212
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J37027
1
BBa_J37027
Pops Blocker (Cre/Lox system)
2006-07-31T11:00:00Z
2015-08-31T04:08:48Z
From Regestry:
BBa_B0015
BBa_I13521
Lox Sites:
Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH
This part is designed to be placed downstream of a promoter and prevent any Pops from the Promoter passing through this part. It will do this until an accompanying Cre Recombinase plasmid becomes activated. Once the Cre recombinase is activated the enzyme produced will permanently cut a section of DNA from the plasmid containing this part. This short section of DNA contains stop codons so once these are removed the polymerase can pass through this part and transcribe downstream genes. This short section of DNA is degraded.
This is useful if a component of the system must be grown up but not activated until a certain external stimulus is added such as a positive feedback loop.
This part should be very efficient at preventing Pops passing through it.
The Part also contains a RFP reporter which is transcribed in the 3???-5??? direction. This means that un-activated parts will fluoresce red and activated parts will not fluoresce. This allows you to see that the part is working in your system. It also allows you to observe the efficiency of activation of the part in your system.
Because of the way Cre recombinase works the excised reporter will remain in a small plasmid and continue to be transcribed for a short time however this plasmid will not have an origin of replication so will not be copied and the fluorescent protein should stop being produced after around 15min
The design uses mutated lox sites, lox66 and lox71, which will make the excision irreversible.
Reference:
Zuwen Zhang and Beat Lutz (2002) Cre Recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein NUCLEAIC ACIDS RESEARCH
false
false
_66_
0
1068
66
It's complicated
true
Used specific lox sites to make this irreversable
true
John Chattaway
annotation1893177
1
BBa_J37026
range1893177
1
1
34
annotation1893182
1
polya
range1893182
1
152
165
annotation1893188
1
Reversed and complementary to part I13521
range1893188
1
168
1091
annotation1893179
1
stem_loop
range1893179
1
48
91
annotation1893184
1
BBa_J37028
range1893184
1
1094
1127
annotation1893180
1
BBa_B0012
range1893180
1
125
165
annotation1893183
1
stop
range1893183
1
158
158
annotation1893178
1
BBa_B0010
range1893178
1
37
116
annotation1893181
1
T7 TE
range1893181
1
132
151
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J37027_sequence
1
cgataacttggtatagcatacattatacgaacggtaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagattataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttctctagtagtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggagttaccgttcgtatacgatacattatacgaagttat
BBa_J37037_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagcgataacttggtatagcatacattatacgaacggtaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagattataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagcgatctacactagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttctctagtagtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggagttaccgttcgtatacgatacattatacgaagttattactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
BBa_B0025_sequence
1
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z