LOCUS BBa_J44007 2587 bp DNA linear 11-May-2024 DEFINITION pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF ACCESSION BBa_J44007 VERSION BBa_J44007.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 misc_feature 299..304 /label=Former SpeI site misc_feature 301..301 /label=Mutation to delete the SpeI site CDS 230..802 /label=Hin sequence primer_bind 230..232 /label=Fwd primer J31001 primer_bind 780..799 /label=Rev primer J31001 CDS 230..802 /label=BBa_J31000 stem_loop 822..865 /label=stem_loop misc_feature 811..890 /label=BBa_B0010 terminator 811..890 /label=BBa_B0010 stem_loop 906..925 /label=T7 TE misc_feature 932..932 /label=stop polyA_site 926..939 /label=polya misc_feature 899..939 /label=BBa_B0012 terminator 899..939 /label=BBa_B0012 misc_feature 948..973 /label=BBa_J44000 promoter 982..1111 /label=promoter promoter 982..1111 /label=BBa_J44002 misc_feature 1120..1145 /label=BBa_J44000 RBS 1154..1168 /label=RBS-1\Strong RBS 1161..1165 /label=RBS misc_feature 1154..1168 /label=BBa_B0030 RBS 1154..1168 /label=BBa_B0030 CDS 1175..2365 /label=tetA(C) forward primer_bind 1175..1198 /label=Fwd primer J31007 primer_bind 2346..2365 /label=Rev primer J31007 misc_feature 2363..2365 /label=stop CDS 1175..2365 /label=BBa_J31007 stem_loop 2385..2428 /label=stem_loop misc_feature 2374..2453 /label=BBa_B0010 terminator 2374..2453 /label=BBa_B0010 stem_loop 2469..2488 /label=T7 TE misc_feature 2495..2495 /label=stop polyA_site 2489..2502 /label=polya misc_feature 2462..2502 /label=BBa_B0012 terminator 2462..2502 /label=BBa_B0012 misc_feature 2561..2566 /label=Former SpeI site misc_feature 2561..2561 /label=Mutation of SpeI site misc_feature 2511..2587 /label=RE Sequence misc_feature 2539..2539 /label=Originally a C misc_feature 2587..2587 /label=Insertion right before biobrick ends misc_feature 2516..2531 /label=Proximal Fis Binding Site misc_feature 2565..2579 /label=Distal Fis Binding Site misc_feature 2511..2587 /label=BBa_J3101 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat 241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 301 cagtgcaaat tgtgaccgca tttttgaaga ccgtatcagt ggcaagattg caaaccgccc 361 cggcctgaaa cgggcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 421 agacagactg ggccgtagcg tgaaaaatct ggtggcgtta atatcagaat tacatgaacg 481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaatcg tcgagcgaac 601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 661 caacaaacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 721 attagctatt atttttggta ttggcgtatc caccttatac agatactttc cggcaagcag 781 tataaaaaaa cgaatgaatt aatactagag ccaggcatca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 901 acactggctc accttcgggt gggcctttct gcgtttatat actagagtta tcaaaaacca 961 tggtttttga taatactaga ggctagccca aaaaaacggt atggagaaac agtagagagt 1021 tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc tatggcatag 1081 caaagtgtga cgccgtgcaa ataatcaatg ttactagagt tatcaaaaac catggttttt 1141 gataatacta gagattaaag aggagaaata ctagatgaaa tctaacaatg cgctcatcgt 1201 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc 1261 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct 1321 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg 1381 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat 1441 catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac 1501 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 1561 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 1621 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 1681 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg 1741 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 1801 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 1861 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 1921 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggccc 1981 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct 2041 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct 2101 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga 2161 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat 2221 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt 2281 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc 2341 atggagccgg gccacctcga cctaatacta gagccaggca tcaaataaaa cgaaaggctc 2401 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2461 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttcgggtgtc 2521 aacaattgac caaaatattg atttacagcg taatgcgctt tctagtgcaa attgtgaccg 2581 cattttg //