LOCUS BBa_J44008 2626 bp DNA linear 13-May-2024 DEFINITION pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF ACCESSION BBa_J44008 VERSION BBa_J44008.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..799 /label=Hin primer_bind 230..232 /label=Fwd primer J31001 misc_feature 800..838 /label=LVA misc_feature 839..841 /label=Stop codon primer_bind 780..841 /label=Rev primer J31001 CDS 230..841 /label=BBa_J31001 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 987..1012 /label=BBa_J44000 promoter 1021..1150 /label=promoter promoter 1021..1150 /label=BBa_J44002 misc_feature 1159..1184 /label=BBa_J44000 RBS 1193..1207 /label=RBS-1\Strong RBS 1200..1204 /label=RBS misc_feature 1193..1207 /label=BBa_B0030 RBS 1193..1207 /label=BBa_B0030 CDS 1214..2404 /label=tetA(C) forward primer_bind 1214..1237 /label=Fwd primer J31007 primer_bind 2385..2404 /label=Rev primer J31007 misc_feature 2402..2404 /label=stop CDS 1214..2404 /label=BBa_J31007 stem_loop 2424..2467 /label=stem_loop misc_feature 2413..2492 /label=BBa_B0010 terminator 2413..2492 /label=BBa_B0010 stem_loop 2508..2527 /label=T7 TE misc_feature 2534..2534 /label=stop polyA_site 2528..2541 /label=polya misc_feature 2501..2541 /label=BBa_B0012 terminator 2501..2541 /label=BBa_B0012 terminator 2413..2541 /label=BBa_B0015 misc_feature 2600..2605 /label=Former SpeI site misc_feature 2600..2600 /label=Mutation of SpeI site misc_feature 2550..2626 /label=RE Sequence misc_feature 2578..2578 /label=Originally a C misc_feature 2626..2626 /label=Insertion right before biobrick ends misc_feature 2555..2570 /label=Proximal Fis Binding Site misc_feature 2604..2618 /label=Distal Fis Binding Site misc_feature 2550..2626 /label=BBa_J3101 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat 241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 301 cagtgcaaat tgtgaccgca tttttgagga ccgtatcagt ggcaagattg caaaccgccc 361 cggcctgaaa cgagcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 421 agacagactg ggccgcagcg tgaaaaacct ggtggcgtta atatcagaat tacatgaacg 481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaattg tcgagcgaac 601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 661 caacagacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 721 actagctatt atttttggta ttggcgtatc taccttatac agatattttc cggcaagccg 781 tataaaaaaa cgaatgaata ggcctgctgc aaacgacgaa aactacgctt tagtagctta 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagttat caaaaaccat ggtttttgat aatactagag 1021 gctagcccaa aaaaacggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta 1081 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa 1141 taatcaatgt tactagagtt atcaaaaacc atggtttttg ataatactag agattaaaga 1201 ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct 1261 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt 1321 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca 1381 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct 1441 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct 1501 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc 1561 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat 1621 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 1681 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact 1741 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc 1801 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 1861 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 1921 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 1981 aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac gtttcggcga 2041 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 2101 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 2161 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 2221 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac 2281 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 2341 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 2401 ctaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2461 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2521 gtgggccttt ctgcgtttat atactagagt tcgggtgtca acaattgacc aaaatattga 2581 tttacagcgt aatgcgcttt ctagtgcaaa ttgtgaccgc attttg //