LOCUS       BBa_J45100              1294 bp    DNA     linear       15-May-2024
DEFINITION  Wintergreen odor enzyme (BSMT1) generator
ACCESSION   BBa_J45100
VERSION     BBa_J45100.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..1157
                     /label=BMST1
     misc_feature    84..86
                     /label=start codon
     misc_feature    1155..1157
                     /label=stop codon
     CDS             84..1157
                     /label=BBa_J45004
     stem_loop       1177..1220
                     /label=stem_loop
     misc_feature    1166..1245
                     /label=BBa_B0010
     terminator      1166..1245
                     /label=BBa_B0010
     stem_loop       1261..1280
                     /label=T7 TE
     misc_feature    1287..1287
                     /label=stop
     polyA_site      1281..1294
                     /label=polya
     misc_feature    1254..1294
                     /label=BBa_B0012
     terminator      1254..1294
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggaag ttgttgaagt tcttcacatg aatggaggaa
      121 atggagacag tagctatgca aacaattctt tggttcagca aaaggtgatt ctcatgacaa
      181 agccaataac tgagcaagcc atgattgatc tctacagcag cctctttcca gaaaccttat
      241 gcattgcaga tttgggttgt tctttgggag ctaacacttt cttggtggtc tcacagcttg
      301 ttaaaatagt agaaaaagaa cgaaaaaagc atggttttaa gtctccagag ttttattttc
      361 acttcaatga tcttcctggc aatgatttta atacactttt tcagtcactg ggggcatttc
      421 aagaagattt gagaaagcat ataggggaaa gctttggtcc atgttttttc agtggagtgc
      481 ctggttcatt ttatactaga cttttccctt ccaaaagttt acattttgtt tactcctcct
      541 acagtctcat gtggctatct caggtgccta atgggattga aaataacaag ggaaacattt
      601 acatggcaag aacaagccct ctaagtgtta ttaaagcata ctacaagcaa tatgaaatag
      661 atttttcaaa ttttctcaag taccgttcag aggaattgat gaaaggtgga aagatggtgt
      721 taacactcct aggtagagaa agtgaggatc ctactagcaa agaatgctgt tacatttggg
      781 agcttctagc catggccctc aataagttgg ttgaagaggg attgataaaa gaagagaaag
      841 tagatgcatt caatattcct caatacacac catcaccagc agaagtaaag tacatagttg
      901 agaaggaagg atcattcacc attaatcgct tggaaacatc aagagttcat tggaatgctt
      961 ctaataatga gaagaatggt ggttacaatg tgtcaaggtg catgagagct gtggctgagc
     1021 ctttgcttgt cagccacttt gacaaggaat tgatggattt agtgttccac aagtacgaag
     1081 agattgtttc tgattgcatg tccaaagaga atactgagtt tataaatgtc atcatctcct
     1141 tgaccaaaat aaattaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1201 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1261 ggctcacctt cgggtgggcc tttctgcgtt tata
//