LOCUS       BBa_J45181              2347 bp    DNA     linear       13-May-2024
DEFINITION  Exponential phase dependent wintergreen odor generator
ACCESSION   BBa_J45181
VERSION     BBa_J45181.1  
FEATURES             Location/Qualifiers
     promoter        188..193
                     /label=-10
     promoter        164..169
                     /label=-35
     promoter        1..199
                     /label=BBa_J45992
     RBS             208..219
                     /label=BBa_B0064
     CDS             229..845
                     /label=tetR
     misc_feature    846..879
                     /label=SsrA
     misc_feature    886..910
                     /label=Help:Barcodes
     CDS             226..910
                     /label=BBa_C0040
     stem_loop       930..973
                     /label=stem_loop
     misc_feature    919..998
                     /label=BBa_B0010
     terminator      919..998
                     /label=BBa_B0010
     stem_loop       1014..1033
                     /label=T7 TE
     misc_feature    1040..1040
                     /label=stop
     polyA_site      1034..1047
                     /label=polya
     misc_feature    1007..1047
                     /label=BBa_B0012
     terminator      1007..1047
                     /label=BBa_B0012
     terminator      919..1047
                     /label=BBa_B0015
     misc_feature    1056..1074
                     /label=TetR 1
     misc_feature    1056..1109
                     /label=BBa_R0040
     promoter        1075..1080
                     /label=-35
     misc_feature    1081..1099
                     /label=TetR 2
     promoter        1098..1103
                     /label=-10
     promoter        1056..1109
                     /label=BBa_R0040
     misc_feature    208..1109
                     /label=BBa_Q04401
     promoter        1..1109
                     /label=BBa_J45994
     RBS             1118..1130
                     /label=RBS-3\Weak
     RBS             1124..1127
                     /label=RBS
     misc_feature    1118..1130
                     /label=BBa_B0032
     RBS             1118..1130
                     /label=BBa_B0032
     CDS             1137..2210
                     /label=BMST1
     misc_feature    1137..1139
                     /label=start codon
     misc_feature    2208..2210
                     /label=stop codon
     CDS             1137..2210
                     /label=BBa_J45004
     stem_loop       2230..2273
                     /label=stem_loop
     misc_feature    2219..2298
                     /label=BBa_B0010
     terminator      2219..2298
                     /label=BBa_B0010
     stem_loop       2314..2333
                     /label=T7 TE
     misc_feature    2340..2340
                     /label=stop
     polyA_site      2334..2347
                     /label=polya
     misc_feature    2307..2347
                     /label=BBa_B0012
     terminator      2307..2347
                     /label=BBa_B0012
     misc_feature    1118..2347
                     /label=BBa_J45119
ORIGIN
        1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg
       61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa
      121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
      181 tgtgatctat atttaacaat actagagaaa gaggggaaat actagatgtc cagattagat
      241 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      301 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      361 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
      421 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
      481 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
      541 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
      601 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
      661 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
      721 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
      781 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
      841 gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag
      901 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      961 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1021 cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca
     1081 tccctatcag tgatagagat actgagcact actagagtca cacaggaaag tactagatgg
     1141 aagttgttga agttcttcac atgaatggag gaaatggaga cagtagctat gcaaacaatt
     1201 ctttggttca gcaaaaggtg attctcatga caaagccaat aactgagcaa gccatgattg
     1261 atctctacag cagcctcttt ccagaaacct tatgcattgc agatttgggt tgttctttgg
     1321 gagctaacac tttcttggtg gtctcacagc ttgttaaaat agtagaaaaa gaacgaaaaa
     1381 agcatggttt taagtctcca gagttttatt ttcacttcaa tgatcttcct ggcaatgatt
     1441 ttaatacact ttttcagtca ctgggggcat ttcaagaaga tttgagaaag catatagggg
     1501 aaagctttgg tccatgtttt ttcagtggag tgcctggttc attttatact agacttttcc
     1561 cttccaaaag tttacatttt gtttactcct cctacagtct catgtggcta tctcaggtgc
     1621 ctaatgggat tgaaaataac aagggaaaca tttacatggc aagaacaagc cctctaagtg
     1681 ttattaaagc atactacaag caatatgaaa tagatttttc aaattttctc aagtaccgtt
     1741 cagaggaatt gatgaaaggt ggaaagatgg tgttaacact cctaggtaga gaaagtgagg
     1801 atcctactag caaagaatgc tgttacattt gggagcttct agccatggcc ctcaataagt
     1861 tggttgaaga gggattgata aaagaagaga aagtagatgc attcaatatt cctcaataca
     1921 caccatcacc agcagaagta aagtacatag ttgagaagga aggatcattc accattaatc
     1981 gcttggaaac atcaagagtt cattggaatg cttctaataa tgagaagaat ggtggttaca
     2041 atgtgtcaag gtgcatgaga gctgtggctg agcctttgct tgtcagccac tttgacaagg
     2101 aattgatgga tttagtgttc cacaagtacg aagagattgt ttctgattgc atgtccaaag
     2161 agaatactga gtttataaat gtcatcatct ccttgaccaa aataaattaa tactagagcc
     2221 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2281 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2341 gtttata
//