LOCUS       BBa_J45200              1801 bp    DNA     linear       15-May-2024
DEFINITION  Banana odor generator
ACCESSION   BBa_J45200
VERSION     BBa_J45200.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..1664
                     /label=ATF1
     misc_feature    1231..1231
                     /label=t -> c
     misc_feature    1659..1664
                     /label=double TAA stop codon
     misc_feature    497..497
                     /label=a->g to eliminate EcoRI site
     CDS             84..1664
                     /label=BBa_J45014
     stem_loop       1684..1727
                     /label=stem_loop
     misc_feature    1673..1752
                     /label=BBa_B0010
     terminator      1673..1752
                     /label=BBa_B0010
     stem_loop       1768..1787
                     /label=T7 TE
     misc_feature    1794..1794
                     /label=stop
     polyA_site      1788..1801
                     /label=polya
     misc_feature    1761..1801
                     /label=BBa_B0012
     terminator      1761..1801
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgaatg aaatcgatga gaaaaatcag gcccccgtgc
      121 aacaagaatg cctgaaagag atgattcaga atgggcatgc tcggcgtatg ggatctgttg
      181 aagatctgta tgttgctctc aacagacaaa acttatatcg aaacttctgc acatatggag
      241 aattgagtga ttactgtact agggatcagc tcacattagc tttgagggaa atctgcctga
      301 aaaatccaac tcttttacat attgttctac caacaagatg gccaaatcat gaaaattatt
      361 atcgcagttc cgaatactat tcacggccac atccagtgca tgattatatt tcagtattac
      421 aagaattgaa actgagtggt gtggttctca atgaacaacc tgagtacagt gcagtaatga
      481 agcaaatatt agaagagttc aaaaatagta agggttccta tactgcaaaa atttttaaac
      541 ttactaccac tttgactatt ccttactttg gaccaacagg accgagttgg cggctaattt
      601 gtcttccaga agagcacaca gaaaagtgga aaaaatttat ctttgtatct aatcattgca
      661 tgtctgatgg tcggtcttcg atccactttt ttcatgattt aagagacgaa ttaaataata
      721 ttaaaactcc accaaaaaaa ttagattaca ttttcaagta cgaggaggat taccaattat
      781 tgaggaaact tccagaaccg atcgaaaagg tgatagactt tagaccaccg tacttgttta
      841 ttccgaagtc acttctttcg ggtttcatct acaatcattt gagattttct tcaaaaggtg
      901 tctgtatgag aatggatgat gtggaaaaaa ccgatgatgt tgtcaccgag atcatcaata
      961 tttcaccaac agaatttcaa gcgattaaag caaatattaa atcaaatatc caaggtaagt
     1021 gtactatcac tccgttttta catgtttgtt ggtttgtatc tcttcataaa tggggtaaat
     1081 ttttcaaacc attgaacttc gaatggctta cggatatttt tatccccgca gattgccgct
     1141 cacaactacc agatgatgat gaaatgagac agatgtacag atatggcgct aacgttggat
     1201 ttattgactt caccccctgg ataagcgaat ctgacatgaa tgataacaaa gaaaattttt
     1261 ggccacttat tgagcactac catgaagtaa tttcggaagc tttaagaaat aaaaagcatc
     1321 tccatggctt agggttcaat atacaaggct tcgttcaaaa atatgtgaac attgacaagg
     1381 taatgtgcga tcgtgccatc gggaaaagac gcggaggtac attgttaagc aatgtaggtc
     1441 tgtttaatca gttagaggag cccgatgcca aatattctat atgcgatttg gcatttggcc
     1501 aatttcaagg atcctggcac caagcatttt ccttgggtgt ttgttcgact aatgtaaagg
     1561 ggatgaatat tgttgttgct tcaacaaaga atgttgttgg tagtcaagaa tctctcgaag
     1621 agctttgctc catttacaaa gctctccttt taggccctta ataatactag agccaggcat
     1681 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1741 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1801 a
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