BBa_J45298 1 BBa_J45298 PchA & PchB enzyme generator 2006-10-27T11:00:00Z 2015-08-31T04:08:49Z coming This part is an intermediate to the protein generator BBa_45300. false false _84_ 0 977 84 It's complicated false coming false Andr?? Green II component1960908 1 BBa_J45017 component1960909 1 BBa_B0010 component1960911 1 BBa_B0012 annotation1960908 1 BBa_J45017 range1960908 1 1 1736 annotation1960911 1 BBa_B0012 range1960911 1 1833 1873 annotation1960909 1 BBa_B0010 range1960909 1 1745 1824 BBa_J45017 1 pchBA isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate 2006-08-20T11:00:00Z 2015-08-31T04:08:49Z pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne. pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB. false false _84_ 0 1147 84 It's complicated true n/a false MIT IGEM 2006 annotation1897457 1 pchA double TAA stop codon range1897457 1 1731 1736 annotation1897454 1 pchA start codon range1897454 1 303 305 annotation1961297 1 PchA range1961297 1 303 1736 annotation1897456 1 pchB stop codon range1897456 1 304 306 annotation1961296 1 PchB range1961296 1 1 306 annotation1897455 1 pchB start codon range1897455 1 1 3 annotation1897453 1 g -> a to eliminate PstI site range1897453 1 440 440 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J45298_sequence 1 atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J45017_sequence 1 atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z