LOCUS       BBa_J45300              1957 bp    DNA     linear       15-May-2024
DEFINITION  Salicylic acid generator
ACCESSION   BBa_J45300
VERSION     BBa_J45300.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     misc_feature    524..524
                     /label=g -> a to eliminate PstI site
     misc_feature    387..389
                     /label=pchA start codon
     misc_feature    85..87
                     /label=pchB start codon
     misc_feature    388..390
                     /label=pchB stop codon
     misc_feature    1815..1820
                     /label=pchA double TAA stop codon
     CDS             85..390
                     /label=PchB
     CDS             387..1820
                     /label=PchA
     CDS             85..1820
                     /label=BBa_J45017
     stem_loop       1840..1883
                     /label=stem_loop
     misc_feature    1829..1908
                     /label=BBa_B0010
     terminator      1829..1908
                     /label=BBa_B0010
     stem_loop       1924..1943
                     /label=T7 TE
     misc_feature    1950..1950
                     /label=stop
     polyA_site      1944..1957
                     /label=polya
     misc_feature    1917..1957
                     /label=BBa_B0012
     terminator      1917..1957
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagattaaag aggagaaata ctagatgaaa actcccgaag actgcaccgg cctggcggac
      121 atccgcgagg ccatcgaccg gatcgacctg gatatcgtcc aggccctcgg ccgccgcatg
      181 gactacgtca aggcggcgtc gcgcttcaag gccagcgagg cggcgattcc ggcgcccgag
      241 cgggtcgccg cgatgctccc cgagcgcgcc cgctgggccg aggaaaacgg actcgacgcg
      301 cccttcgtcg agggactgtt cgcgcagatc atccactggt acatcgccga gcagatcaag
      361 tactggcgcc agacacgggg tgccgcatga gccggctggc gcccctgagc cagtgcctgc
      421 acgccttgcg cggcaccttc gagcgcgcca tcggccaggc gcaggcgctc gatcgtccgg
      481 tgctggtggc ggcatcgttc gagatcgacc cattggaccc gctacaggta ttcggtgcct
      541 gggacgaccg gcaaacgccc tgcctgtact gggaacagcc cgagctggcg ttcttcgcct
      601 ggggctgcgc cctggagctg caaggccacg gcgaacagcg cttcgcccgg atcgaggaaa
      661 actggcaatt gctctgcgcc gacgccgtgg tcgagggccc gctggcgccg cgcctgtgcg
      721 gcggattccg cttcgatccg cgcggcccgc gcgaggaaca ctggcaagcc ttcgccgatg
      781 ccagcctgat gctcgccggc atcaccgtgc tgcgcgaggg cgaacgctac cgggtactct
      841 gccaacacct ggccaagccc ggcgaagatg ccctggccct ggccgcctac cactgctcgg
      901 cgctactgcg cctgaggcag ccggccagac gccggccctc ggggccgacc gctggcgcgc
      961 agggcgacgc ttcggcgcag gagcgcaggc aatgggaagc caaggtgagc gacgcggtaa
     1021 gcagtgtccg ccagggacgc ttcggcaagg tcgtgctggc ccgcacccag gcccggcctc
     1081 tcggcgacat cgagccgtgg caggtcatcg aacacctgcg tctgcaacat gccgacgccc
     1141 agctgttcgc ctgtcgccgc ggcaacgcct gcttcctcgg cgcctccccg gaacgcctgg
     1201 tccgcattcg cgccggcgag gcactcaccc atgccctggc cgggaccatc gcccgcggcg
     1261 gcgatgccca ggaagatgcg cggctcggac aggccctgct ggacagcgcc aaggacaggc
     1321 acgaacacca gttggtggtg gaggcgatcc gtacggccct ggaacccttc agcgaggtgc
     1381 tggaaatccc cgatgcgccc ggcctgaaac gactggcgcg agtccagcac ctgaacacgc
     1441 cgatccgcgc ccgcctcgct gacgcaggcg gcatcctgcg gctgctacaa gcgctgcatc
     1501 cgacccccgc ggtgggcggc tacccacgca gcgcggcgct ggactacatc cgccagcacg
     1561 aagggatgga ccgcggctgg tacgccgcgc cgctgggctg gctcgacggc gaaggcaacg
     1621 gcgatttcct ggtggcgctg cgctcggccc tgctcacgcc gggccggggc tacctgttcg
     1681 ccggctgcgg tctggtaggc gattcggaac cggcccacga gtatcgcgaa acctgcctta
     1741 agctcagtgc catgcgggaa gctctatccg ccataggcgg cctggacgaa gtgcccttgc
     1801 agcgcggcgt cgcctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1861 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1921 actggctcac cttcgggtgg gcctttctgc gtttata
//