LOCUS       BBa_J45319              1892 bp    DNA     linear       19-Apr-2024
DEFINITION  PchA & PchB enzyme generator
ACCESSION   BBa_J45319
VERSION     BBa_J45319.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    459..459
                     /label=g -> a to eliminate PstI site
     misc_feature    322..324
                     /label=pchA start codon
     misc_feature    20..22
                     /label=pchB start codon
     misc_feature    323..325
                     /label=pchB stop codon
     misc_feature    1750..1755
                     /label=pchA double TAA stop codon
     CDS             20..325
                     /label=PchB
     CDS             322..1755
                     /label=PchA
     CDS             20..1755
                     /label=BBa_J45017
     stem_loop       1775..1818
                     /label=stem_loop
     misc_feature    1764..1843
                     /label=BBa_B0010
     terminator      1764..1843
                     /label=BBa_B0010
     stem_loop       1859..1878
                     /label=T7 TE
     misc_feature    1885..1885
                     /label=stop
     polyA_site      1879..1892
                     /label=polya
     misc_feature    1852..1892
                     /label=BBa_B0012
     terminator      1852..1892
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgaaaactcc cgaagactgc accggcctgg cggacatccg
       61 cgaggccatc gaccggatcg acctggatat cgtccaggcc ctcggccgcc gcatggacta
      121 cgtcaaggcg gcgtcgcgct tcaaggccag cgaggcggcg attccggcgc ccgagcgggt
      181 cgccgcgatg ctccccgagc gcgcccgctg ggccgaggaa aacggactcg acgcgccctt
      241 cgtcgaggga ctgttcgcgc agatcatcca ctggtacatc gccgagcaga tcaagtactg
      301 gcgccagaca cggggtgccg catgagccgg ctggcgcccc tgagccagtg cctgcacgcc
      361 ttgcgcggca ccttcgagcg cgccatcggc caggcgcagg cgctcgatcg tccggtgctg
      421 gtggcggcat cgttcgagat cgacccattg gacccgctac aggtattcgg tgcctgggac
      481 gaccggcaaa cgccctgcct gtactgggaa cagcccgagc tggcgttctt cgcctggggc
      541 tgcgccctgg agctgcaagg ccacggcgaa cagcgcttcg cccggatcga ggaaaactgg
      601 caattgctct gcgccgacgc cgtggtcgag ggcccgctgg cgccgcgcct gtgcggcgga
      661 ttccgcttcg atccgcgcgg cccgcgcgag gaacactggc aagccttcgc cgatgccagc
      721 ctgatgctcg ccggcatcac cgtgctgcgc gagggcgaac gctaccgggt actctgccaa
      781 cacctggcca agcccggcga agatgccctg gccctggccg cctaccactg ctcggcgcta
      841 ctgcgcctga ggcagccggc cagacgccgg ccctcggggc cgaccgctgg cgcgcagggc
      901 gacgcttcgg cgcaggagcg caggcaatgg gaagccaagg tgagcgacgc ggtaagcagt
      961 gtccgccagg gacgcttcgg caaggtcgtg ctggcccgca cccaggcccg gcctctcggc
     1021 gacatcgagc cgtggcaggt catcgaacac ctgcgtctgc aacatgccga cgcccagctg
     1081 ttcgcctgtc gccgcggcaa cgcctgcttc ctcggcgcct ccccggaacg cctggtccgc
     1141 attcgcgccg gcgaggcact cacccatgcc ctggccggga ccatcgcccg cggcggcgat
     1201 gcccaggaag atgcgcggct cggacaggcc ctgctggaca gcgccaagga caggcacgaa
     1261 caccagttgg tggtggaggc gatccgtacg gccctggaac ccttcagcga ggtgctggaa
     1321 atccccgatg cgcccggcct gaaacgactg gcgcgagtcc agcacctgaa cacgccgatc
     1381 cgcgcccgcc tcgctgacgc aggcggcatc ctgcggctgc tacaagcgct gcatccgacc
     1441 cccgcggtgg gcggctaccc acgcagcgcg gcgctggact acatccgcca gcacgaaggg
     1501 atggaccgcg gctggtacgc cgcgccgctg ggctggctcg acggcgaagg caacggcgat
     1561 ttcctggtgg cgctgcgctc ggccctgctc acgccgggcc ggggctacct gttcgccggc
     1621 tgcggtctgg taggcgattc ggaaccggcc cacgagtatc gcgaaacctg ccttaagctc
     1681 agtgccatgc gggaagctct atccgccata ggcggcctgg acgaagtgcc cttgcagcgc
     1741 ggcgtcgcct aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1801 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1861 ctcaccttcg ggtgggcctt tctgcgttta ta
//