LOCUS       BBa_J45320              1955 bp    DNA     linear       29-May-2024
DEFINITION  Salicylate generator
ACCESSION   BBa_J45320
VERSION     BBa_J45320.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     misc_feature    522..522
                     /label=g -> a to eliminate PstI site
     misc_feature    385..387
                     /label=pchA start codon
     misc_feature    83..85
                     /label=pchB start codon
     misc_feature    386..388
                     /label=pchB stop codon
     misc_feature    1813..1818
                     /label=pchA double TAA stop codon
     CDS             83..388
                     /label=PchB
     CDS             385..1818
                     /label=PchA
     CDS             83..1818
                     /label=BBa_J45017
     stem_loop       1838..1881
                     /label=stem_loop
     misc_feature    1827..1906
                     /label=BBa_B0010
     terminator      1827..1906
                     /label=BBa_B0010
     stem_loop       1922..1941
                     /label=T7 TE
     misc_feature    1948..1948
                     /label=stop
     polyA_site      1942..1955
                     /label=polya
     misc_feature    1915..1955
                     /label=BBa_B0012
     terminator      1915..1955
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgaaaac tcccgaagac tgcaccggcc tggcggacat
      121 ccgcgaggcc atcgaccgga tcgacctgga tatcgtccag gccctcggcc gccgcatgga
      181 ctacgtcaag gcggcgtcgc gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg
      241 ggtcgccgcg atgctccccg agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc
      301 cttcgtcgag ggactgttcg cgcagatcat ccactggtac atcgccgagc agatcaagta
      361 ctggcgccag acacggggtg ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac
      421 gccttgcgcg gcaccttcga gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg
      481 ctggtggcgg catcgttcga gatcgaccca ttggacccgc tacaggtatt cggtgcctgg
      541 gacgaccggc aaacgccctg cctgtactgg gaacagcccg agctggcgtt cttcgcctgg
      601 ggctgcgccc tggagctgca aggccacggc gaacagcgct tcgcccggat cgaggaaaac
      661 tggcaattgc tctgcgccga cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc
      721 ggattccgct tcgatccgcg cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc
      781 agcctgatgc tcgccggcat caccgtgctg cgcgagggcg aacgctaccg ggtactctgc
      841 caacacctgg ccaagcccgg cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg
      901 ctactgcgcc tgaggcagcc ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag
      961 ggcgacgctt cggcgcagga gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc
     1021 agtgtccgcc agggacgctt cggcaaggtc gtgctggccc gcacccaggc ccggcctctc
     1081 ggcgacatcg agccgtggca ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag
     1141 ctgttcgcct gtcgccgcgg caacgcctgc ttcctcggcg cctccccgga acgcctggtc
     1201 cgcattcgcg ccggcgaggc actcacccat gccctggccg ggaccatcgc ccgcggcggc
     1261 gatgcccagg aagatgcgcg gctcggacag gccctgctgg acagcgccaa ggacaggcac
     1321 gaacaccagt tggtggtgga ggcgatccgt acggccctgg aacccttcag cgaggtgctg
     1381 gaaatccccg atgcgcccgg cctgaaacga ctggcgcgag tccagcacct gaacacgccg
     1441 atccgcgccc gcctcgctga cgcaggcggc atcctgcggc tgctacaagc gctgcatccg
     1501 acccccgcgg tgggcggcta cccacgcagc gcggcgctgg actacatccg ccagcacgaa
     1561 gggatggacc gcggctggta cgccgcgccg ctgggctggc tcgacggcga aggcaacggc
     1621 gatttcctgg tggcgctgcg ctcggccctg ctcacgccgg gccggggcta cctgttcgcc
     1681 ggctgcggtc tggtaggcga ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag
     1741 ctcagtgcca tgcgggaagc tctatccgcc ataggcggcc tggacgaagt gcccttgcag
     1801 cgcggcgtcg cctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1921 tggctcacct tcgggtgggc ctttctgcgt ttata
//