LOCUS       BBa_J45398              3014 bp    DNA     linear       17-May-2024
DEFINITION  BAT2 & THI3 enzyme generator
ACCESSION   BBa_J45398
VERSION     BBa_J45398.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start codon
     misc_feature    1150..1155
                     /label=double TAA stop codon
     misc_feature    792..792
                     /label=a->g to eliminate SpeI
     CDS             22..1155
                     /label=BAT2
     CDS             22..1155
                     /label=BBa_J45008
     RBS             1164..1178
                     /label=RBS-1\Strong
     RBS             1171..1175
                     /label=RBS
     misc_feature    1164..1178
                     /label=BBa_B0030
     RBS             1164..1178
                     /label=BBa_B0030
     misc_feature    1190..1190
                     /label=t->c to eliminate EcoRI, to do
     misc_feature    2090..2090
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    2789..2789
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    2975..2975
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    1883..1883
                     /label=t->c to eliminate SpeI, to do
     CDS             1185..3014
                     /label=THI3
     misc_feature    1185..1187
                     /label=start codon
     misc_feature    3012..3014
                     /label=stop codon
     CDS             1185..3014
                     /label=BBa_J45009
ORIGIN
        1 attaaagagg agaaatacta gatgaccttg gcacccctag acgcctccaa agttaagata
       61 actaccacac aacatgcatc taagccaaaa ccgaacagtg agttagtgtt tggcaagagc
      121 ttcacggacc acatgttaac tgcggaatgg acagctgaaa aagggtgggg taccccagag
      181 attaaacctt atcaaaatct gtctttagac ccttccgcgg tggttttcca ttatgctttt
      241 gagctattcg aagggatgaa ggcttacaga acggtggaca acaaaattac aatgtttcgt
      301 ccagatatga atatgaagcg catgaataag tctgctcaga gaatctgttt gccaacgttc
      361 gacccagaag agttgattac cctaattggg aaactgatcc agcaagataa gtgcttagtt
      421 cctgaaggaa aaggttactc tttatatatc aggcctacat taatcggcac tacggccggt
      481 ttaggggttt ccacgcctga tagagccttg ctatatgtca tttgctgccc tgtgggtcct
      541 tattacaaaa ctggatttaa ggcggtcaga ctggaagcca ctgattatgc cacaagagct
      601 tggccaggag gctgtggtga caagaaacta ggtgcaaact acgccccctg cgtcctgcca
      661 caattgcaag ctgcttcaag gggttaccaa caaaatttat ggctatttgg tccaaataac
      721 aacattactg aagtcggcac catgaatgct tttttcgtgt ttaaagatag taaaacgggc
      781 aagaaggaac tggttactgc tccactagac ggtaccattt tggaaggtgt tactagggat
      841 tccattttaa atcttgctaa agaaagactc gaaccaagtg aatggaccat tagtgaacgc
      901 tacttcacta taggcgaagt tactgagaga tccaagaacg gtgaactact tgaagccttt
      961 ggttctggta ctgctgcgat tgtttctccc attaaggaaa tcggctggaa aggcgaacaa
     1021 attaatattc cgttgttgcc cggcgaacaa accggtccat tggccaaaga agttgcacaa
     1081 tggattaatg gaatccaata tggcgagact gagcatggca attggtcaag ggttgttact
     1141 gatttgaact aataatacta gagattaaag aggagaaata ctagatgaat tctagctata
     1201 cacagagata tgcactgccg aagtgtatag caatatcaga ttatcttttc catcggctca
     1261 accagctgaa catacatacc atatttggac tctccggaga atttagcatg ccgttgctgg
     1321 ataaactata caacattccg aacttacgat gggccggtaa ttctaatgag ttaaatgctg
     1381 cctacgcagc agatggatac tcacgactaa aaggcttggg atgtctcata acaacctttg
     1441 gtgtaggcga attatcggca atcaatggcg tggccggatc ttacgctgaa catgtaggaa
     1501 tacttcacat agtgggtatg ccgccaacaa gtgcacaaac gaaacaacta ctactgcatc
     1561 atactctggg caatggtgat ttcacggtat ttcatagaat agccagtgat gtagcatgct
     1621 atacaacatt gattattgac tctgaattat gtgccgacga agtcgataag tgcatcaaaa
     1681 aggcttggat agaacagagg ccagtataca tgggcatgcc tgtcaaccag gtaaatctcc
     1741 cgattgaatc agcaaggctt aatacacctc tggatttaca attgcataaa aacgacccag
     1801 acgtagagaa agaagttatt tctcgaatat tgagttttat atacaaaagc cagaatccgg
     1861 caatcatcgt agatgcatgt actagtcgac agaatttaat cgaggagact aaagagcttt
     1921 gtaataggct taaatttcca gtttttgtta cacctatggg taagggtaca gtaaacgaaa
     1981 cagacccgca atttgggggc gtattcacgg gctcgatatc agccccagaa gtaagagaag
     2041 tagttgattt tgccgatttt atcatcgtca ttggttgcat gctctccgaa ttcagcacgt
     2101 caactttcca cttccaatat aaaactaaga attgtgcgct actatattct acatctgtga
     2161 aattgaaaaa tgccacatat cctgacttga gcattaaatt actactacag aaaatattag
     2221 caaatcttga tgaatctaaa ctgtcttacc aaccaagcga acaacccagt atgatggttc
     2281 caagacctta cccagcagga aatgtcctct tgagacaaga atgggtctgg aatgaaatat
     2341 cccattggtt ccaaccaggt gacataatca taacagaaac tggtgcttct gcatttggag
     2401 ttaaccagac cagatttccg gtaaatacac taggtatttc gcaagctctt tggggatctg
     2461 tcggatatac aatgggggcg tgtcttgggg cagaatttgc tgttcaagag ataaacaagg
     2521 ataaattccc cgcaactaaa catagagtta ttctgtttat gggtgacggt gctttccaat
     2581 tgacagttca agaattatcc acaattgtta agtggggatt gacaccttat atttttgtga
     2641 tgaataacca aggttactct gtggacaggt ttttgcatca caggtcagat gctagttatt
     2701 acgatatcca accttggaac tacttgggat tattgcgagt atttggttgc acgaactacg
     2761 aaacgaaaaa aattattact gttggagaat tcagatccat gatcagtgac ccaaactttg
     2821 cgaccaatga caaaattcgg atgatagaga ttatgctacc accaagggat gttccacagg
     2881 ctctgcttga caggtgggtg gtagaaaaag aacagagcaa acaagtgcaa gaggagaacg
     2941 aaaattctag cgcagtaaat acgccaactc cagaattcca accacttcta aaaaaaaatc
     3001 aagttggata ctga
//