LOCUS       BBa_J45400              3077 bp    DNA     linear       16-May-2024
DEFINITION  3-methylbutanal generator
ACCESSION   BBa_J45400
VERSION     BBa_J45400.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     misc_feature    85..87
                     /label=start codon
     misc_feature    1213..1218
                     /label=double TAA stop codon
     misc_feature    855..855
                     /label=a->g to eliminate SpeI
     CDS             85..1218
                     /label=BAT2
     CDS             85..1218
                     /label=BBa_J45008
     RBS             1227..1241
                     /label=RBS-1\Strong
     RBS             1234..1238
                     /label=RBS
     misc_feature    1227..1241
                     /label=BBa_B0030
     RBS             1227..1241
                     /label=BBa_B0030
     misc_feature    1253..1253
                     /label=t->c to eliminate EcoRI, to do
     misc_feature    2153..2153
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    2852..2852
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    3038..3038
                     /label=a->g to eliminate EcoRI, to do
     misc_feature    1946..1946
                     /label=t->c to eliminate SpeI, to do
     CDS             1248..3077
                     /label=THI3
     misc_feature    1248..1250
                     /label=start codon
     misc_feature    3075..3077
                     /label=stop codon
     CDS             1248..3077
                     /label=BBa_J45009
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagattaaag aggagaaata ctagatgacc ttggcacccc tagacgcctc caaagttaag
      121 ataactacca cacaacatgc atctaagcca aaaccgaaca gtgagttagt gtttggcaag
      181 agcttcacgg accacatgtt aactgcggaa tggacagctg aaaaagggtg gggtacccca
      241 gagattaaac cttatcaaaa tctgtcttta gacccttccg cggtggtttt ccattatgct
      301 tttgagctat tcgaagggat gaaggcttac agaacggtgg acaacaaaat tacaatgttt
      361 cgtccagata tgaatatgaa gcgcatgaat aagtctgctc agagaatctg tttgccaacg
      421 ttcgacccag aagagttgat taccctaatt gggaaactga tccagcaaga taagtgctta
      481 gttcctgaag gaaaaggtta ctctttatat atcaggccta cattaatcgg cactacggcc
      541 ggtttagggg tttccacgcc tgatagagcc ttgctatatg tcatttgctg ccctgtgggt
      601 ccttattaca aaactggatt taaggcggtc agactggaag ccactgatta tgccacaaga
      661 gcttggccag gaggctgtgg tgacaagaaa ctaggtgcaa actacgcccc ctgcgtcctg
      721 ccacaattgc aagctgcttc aaggggttac caacaaaatt tatggctatt tggtccaaat
      781 aacaacatta ctgaagtcgg caccatgaat gcttttttcg tgtttaaaga tagtaaaacg
      841 ggcaagaagg aactggttac tgctccacta gacggtacca ttttggaagg tgttactagg
      901 gattccattt taaatcttgc taaagaaaga ctcgaaccaa gtgaatggac cattagtgaa
      961 cgctacttca ctataggcga agttactgag agatccaaga acggtgaact acttgaagcc
     1021 tttggttctg gtactgctgc gattgtttct cccattaagg aaatcggctg gaaaggcgaa
     1081 caaattaata ttccgttgtt gcccggcgaa caaaccggtc cattggccaa agaagttgca
     1141 caatggatta atggaatcca atatggcgag actgagcatg gcaattggtc aagggttgtt
     1201 actgatttga actaataata ctagagatta aagaggagaa atactagatg aattctagct
     1261 atacacagag atatgcactg ccgaagtgta tagcaatatc agattatctt ttccatcggc
     1321 tcaaccagct gaacatacat accatatttg gactctccgg agaatttagc atgccgttgc
     1381 tggataaact atacaacatt ccgaacttac gatgggccgg taattctaat gagttaaatg
     1441 ctgcctacgc agcagatgga tactcacgac taaaaggctt gggatgtctc ataacaacct
     1501 ttggtgtagg cgaattatcg gcaatcaatg gcgtggccgg atcttacgct gaacatgtag
     1561 gaatacttca catagtgggt atgccgccaa caagtgcaca aacgaaacaa ctactactgc
     1621 atcatactct gggcaatggt gatttcacgg tatttcatag aatagccagt gatgtagcat
     1681 gctatacaac attgattatt gactctgaat tatgtgccga cgaagtcgat aagtgcatca
     1741 aaaaggcttg gatagaacag aggccagtat acatgggcat gcctgtcaac caggtaaatc
     1801 tcccgattga atcagcaagg cttaatacac ctctggattt acaattgcat aaaaacgacc
     1861 cagacgtaga gaaagaagtt atttctcgaa tattgagttt tatatacaaa agccagaatc
     1921 cggcaatcat cgtagatgca tgtactagtc gacagaattt aatcgaggag actaaagagc
     1981 tttgtaatag gcttaaattt ccagtttttg ttacacctat gggtaagggt acagtaaacg
     2041 aaacagaccc gcaatttggg ggcgtattca cgggctcgat atcagcccca gaagtaagag
     2101 aagtagttga ttttgccgat tttatcatcg tcattggttg catgctctcc gaattcagca
     2161 cgtcaacttt ccacttccaa tataaaacta agaattgtgc gctactatat tctacatctg
     2221 tgaaattgaa aaatgccaca tatcctgact tgagcattaa attactacta cagaaaatat
     2281 tagcaaatct tgatgaatct aaactgtctt accaaccaag cgaacaaccc agtatgatgg
     2341 ttccaagacc ttacccagca ggaaatgtcc tcttgagaca agaatgggtc tggaatgaaa
     2401 tatcccattg gttccaacca ggtgacataa tcataacaga aactggtgct tctgcatttg
     2461 gagttaacca gaccagattt ccggtaaata cactaggtat ttcgcaagct ctttggggat
     2521 ctgtcggata tacaatgggg gcgtgtcttg gggcagaatt tgctgttcaa gagataaaca
     2581 aggataaatt ccccgcaact aaacatagag ttattctgtt tatgggtgac ggtgctttcc
     2641 aattgacagt tcaagaatta tccacaattg ttaagtgggg attgacacct tatatttttg
     2701 tgatgaataa ccaaggttac tctgtggaca ggtttttgca tcacaggtca gatgctagtt
     2761 attacgatat ccaaccttgg aactacttgg gattattgcg agtatttggt tgcacgaact
     2821 acgaaacgaa aaaaattatt actgttggag aattcagatc catgatcagt gacccaaact
     2881 ttgcgaccaa tgacaaaatt cggatgatag agattatgct accaccaagg gatgttccac
     2941 aggctctgct tgacaggtgg gtggtagaaa aagaacagag caaacaagtg caagaggaga
     3001 acgaaaattc tagcgcagta aatacgccaa ctccagaatt ccaaccactt ctaaaaaaaa
     3061 atcaagttgg atactga
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