LOCUS       BBa_J45995              1085 bp    DNA     linear       19-May-2024
DEFINITION  Stationary phase dependent GFP generator
ACCESSION   BBa_J45995
VERSION     BBa_J45995.1  
FEATURES             Location/Qualifiers
     promoter        188..193
                     /label=-10
     promoter        164..169
                     /label=-35
     promoter        1..199
                     /label=BBa_J45992
     RBS             208..222
                     /label=RBS-1\Strong
     RBS             215..219
                     /label=RBS
     misc_feature    208..222
                     /label=BBa_B0030
     RBS             208..222
                     /label=BBa_B0030
     CDS             229..948
                     /label=GFP protein
     CDS             229..948
                     /label=BBa_E0040
     stem_loop       968..1011
                     /label=stem_loop
     misc_feature    957..1036
                     /label=BBa_B0010
     terminator      957..1036
                     /label=BBa_B0010
     stem_loop       1052..1071
                     /label=T7 TE
     misc_feature    1078..1078
                     /label=stop
     polyA_site      1072..1085
                     /label=polya
     misc_feature    1045..1085
                     /label=BBa_B0012
     terminator      1045..1085
                     /label=BBa_B0012
ORIGIN
        1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg
       61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa
      121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
      181 tgtgatctat atttaacaat actagagatt aaagaggaga aatactagat gcgtaaagga
      241 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      301 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      361 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      421 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      481 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      541 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      601 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      661 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      721 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      781 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      841 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      901 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
      961 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1021 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1081 ttata
//