LOCUS       BBa_J47052              1355 bp    DNA     linear       15-May-2024
DEFINITION  Constitutive device for lacI repressor, medium RBS
ACCESSION   BBa_J47052
VERSION     BBa_J47052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    52..55
                     /label=conserved
     RBS             46..59
                     /label=BBa_B0031
     misc_feature    1155..1193
                     /label=LVA
     CDS             66..1193
                     /label=lacI-LVA
     misc_feature    66..1193
                     /label=BBa_C0012
     misc_feature    1194..1218
                     /label=Help:Barcodes
     CDS             66..1193
                     /label=BBa_C0012
     stem_loop       1238..1281
                     /label=stem_loop
     misc_feature    1227..1306
                     /label=BBa_B0010
     terminator      1227..1306
                     /label=BBa_B0010
     stem_loop       1322..1341
                     /label=T7 TE
     misc_feature    1348..1348
                     /label=stop
     polyA_site      1342..1355
                     /label=polya
     misc_feature    1315..1355
                     /label=BBa_B0012
     terminator      1315..1355
                     /label=BBa_B0012
     terminator      1227..1355
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagtcaca caggaaacct
       61 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
      121 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
      181 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
      241 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
      301 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
      361 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
      421 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
      481 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
      541 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
      601 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
      661 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
      721 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
      781 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
      841 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
      901 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
      961 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     1021 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     1081 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     1141 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat
     1201 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1261 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1321 tggctcacct tcgggtgggc ctttctgcgt ttata
//