LOCUS       BBa_J47056              1364 bp    DNA     linear       15-May-2024
DEFINITION  Constitutive device (medium transcription)  for lacI repressor, 
            strong RBS
ACCESSION   BBa_J47056
VERSION     BBa_J47056.1  
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label=P(Kat)
     promoter        1..45
                     /label=BBa_I14034
     RBS             54..68
                     /label=RBS-1\Strong
     RBS             61..65
                     /label=RBS
     misc_feature    54..68
                     /label=BBa_B0030
     RBS             54..68
                     /label=BBa_B0030
     misc_feature    1164..1202
                     /label=LVA
     CDS             75..1202
                     /label=lacI-LVA
     misc_feature    75..1202
                     /label=BBa_C0012
     misc_feature    1203..1227
                     /label=Help:Barcodes
     CDS             75..1202
                     /label=BBa_C0012
     stem_loop       1247..1290
                     /label=stem_loop
     misc_feature    1236..1315
                     /label=BBa_B0010
     terminator      1236..1315
                     /label=BBa_B0010
     stem_loop       1331..1350
                     /label=T7 TE
     misc_feature    1357..1357
                     /label=stop
     polyA_site      1351..1364
                     /label=polya
     misc_feature    1324..1364
                     /label=BBa_B0012
     terminator      1324..1364
                     /label=BBa_B0012
     terminator      1236..1364
                     /label=BBa_B0015
ORIGIN
        1 cattattgca attaataaac aactaacgga caattctacc taacatacta gagattaaag
       61 aggagaaata ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
      121 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
      181 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
      241 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
      301 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
      361 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
      421 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
      481 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
      541 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
      601 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
      661 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
      721 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
      781 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
      841 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
      901 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
      961 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     1021 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     1081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1141 tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1201 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc
     1261 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1321 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//