BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J47425
1
BBa_J47425
constitutive luxI sender device
2006-10-26T11:00:00Z
2015-08-31T04:08:50Z
This part was contstructed from standard registry biobricks.
I14033.B0030.C0161
false
false
_72_40_
0
475
72
Not in stock
false
Sender device parts were constructed combinatorially with three different consitutive promoters (I14032, I14033, I14034), two different ribosome binding sites (B0030, B0031), and luxI with or without a degradation tag (C0061, C0161).
false
Jon Badalamenti
component1907802
1
BBa_B0030
component1907800
1
BBa_I14033
component1907805
1
BBa_C0161
annotation1907800
1
BBa_I14033
range1907800
1
1
38
annotation1907805
1
BBa_C0161
range1907805
1
68
652
annotation1907802
1
BBa_B0030
range1907802
1
47
61
BBa_C0161
1
luxI
autoinducer synthetase for AHL (no LVA)
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
Released HQ 2013
same as C0061 except no LVA tag
false
true
_11_1_
0
61
7
In stock
false
true
jcbraff
annotation1891615
1
LuxI
range1891615
1
1
585
BBa_I14033
1
Cat
P(Cat)
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pACYC184
Released HQ 2013
Constitutive Promoter, Medium Transcription
false
false
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1026174
1
P(Cat)
range1026174
1
1
38
annotation1026173
1
-10
range1026173
1
27
32
annotation1026172
1
-35
range1026172
1
4
9
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_C0161_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataa
BBa_I14033_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaaca
BBa_J47425_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaacatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z