BBa_J52026

BBa_J52026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J52026
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jelka Pohar
Date created: 2006-10-14 11:00:00
Date modified: 2016-01-26 09:39:38

dnMyD88-linker-GFP



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_J52026_sequence (Version 1)

    Description

    dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.

    Notes

    dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.

    Source

    dnMyD88-
    GFP-
    more to come

    Sequence Annotation Location Component / Role(s)
    dnMyD88
    linker
    GFP
    dnMyD88-linker-GFP
    		1,417
    418,435
    436,1155
    1,1155
    		feature/cds CDS
    feature/protein CDS
    feature/cds CDS
    engineered_region feature/BioBrick

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J52026/1
     

    Attachments

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