LOCUS       BBa_J52029              2041 bp    DNA     linear       19-Apr-2024
DEFINITION  NF-kappaB + GFP-PEST191
ACCESSION   BBa_J52029
VERSION     BBa_J52029.1  
FEATURES             Location/Qualifiers
     promoter        22..814
                     /label=NFkappaB
     misc_feature    1..814
                     /label=BioBrick
     primer_bind     813..855
                     /label=SI42
     primer_bind     16..43
                     /label=SI41
     promoter        1..814
                     /label=BBa_J52010
     CDS             821..1537
                     /label=GFP
     CDS             1538..1555
                     /label=linker
     misc_feature    1556..2041
                     /label=PEST191 (degradation flag)
     misc_feature    821..2041
                     /label=GFP-linker-PEST191
     misc_feature    821..823
                     /label=start codon
     misc_feature    2039..2041
                     /label=stop codon
     misc_feature    821..2041
                     /label=BBa_J52028
ORIGIN
        1 ccaaagtggt gggattacag gcgtgagcca ctgtgcctgg cctccttttt atttttttca
       61 ctgaacaaac catgaaactt tcccagatgt aaatatctat ttcccatttt tcttttttta
      121 aaataaggca ttattttaac catttgagtg ttagatatta tttttagata atattttaat
      181 ttaggcataa ctgccgtgca aaatctgaag attaatatct accttgtgag tcattcctct
      241 gtgagacagt gcatgttaaa tatgttgaat tggcaggtga aaaaggaaga aaaaatgagt
      301 agtgattggt tatccacagc tatgaatgag aaattgaagg tagtagacta tggatgacaa
      361 acctattctt ggtttccttc tgtttctgaa attctaatta ctaccacaac tacatgagag
      421 acactactaa caagcaaagt tttacaactt tttaaagaca tagactttat gttattataa
      481 ttaaaaatca tgcatttttg tcatattaat aaaattgcat atacgatata aaggcatgga
      541 caaaggtgaa gtagcttcaa gagacagagt ttctgacatc attgtaattt taagcatcgt
      601 ggatattccc gggaaagttt ttggatgcca ttggggattt cctctttact ggatgtggac
      661 aatatcctcc tattattcac aggaagcggc ggccgccata aaagggcctc agccgaagta
      721 gtgttcagct gttcttggct gacttcacat caaaactcct atactgacct gagacagatc
      781 taagtaagct tggcattccg gtactgttgg taaatactag atggtgagca agggcgagga
      841 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      901 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      961 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg
     1021 gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1081 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1141 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1201 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat
     1261 cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     1321 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1381 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1441 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1501 cgccgggatc actctcggca tggacgagct gtacaagggt ggttctggtg gtactgctgc
     1561 ggtgctgacc ttggccgtgc tcttcctgac ggggagccag gctcggcatt tctggcagca
     1621 agatgaaccc ccccagagcc cctgggatcg agtgaaggac ctggccactg tgtacgtgga
     1681 tgtgctcaaa gacagcggca gagactatgt gtcccagttt gaaggctccg ccttgggaaa
     1741 acagctaaac ctaaagctcc ttgacaactg ggacagcgtg acctccacct tcagcaagct
     1801 gcgcgaacag ctcggccctg tgacccagga gttctgggat aacctggaaa aggagacaga
     1861 gggcctgagg caggagatga gcaaggatct ggaggaggtg aaggccaagg tgcagcccta
     1921 cctggacgac ttccagaaga agtggcagga ggagatggag ctctaccgcc agaaggtgga
     1981 gccgctgcgc gcagagctcc aagagggcgc gcgccagaag ctgcacgagc tgcaagagta
     2041 g
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