LOCUS       BBa_J52040              1881 bp    DNA     linear       03-May-2024
DEFINITION  CMV + GFP-PEST191
ACCESSION   BBa_J52040
VERSION     BBa_J52040.1  
FEATURES             Location/Qualifiers
     promoter        1..654
                     /label=CMV+RBS
     promoter        1..654
                     /label=BBa_J52034
     CDS             661..1377
                     /label=GFP
     CDS             1378..1395
                     /label=linker
     misc_feature    1396..1881
                     /label=PEST191 (degradation flag)
     misc_feature    661..1881
                     /label=GFP-linker-PEST191
     misc_feature    661..663
                     /label=start codon
     misc_feature    1879..1881
                     /label=stop codon
     misc_feature    661..1881
                     /label=BBa_J52028
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattactag
      661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      841 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1081 aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     1141 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1261 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     1381 ggttctggtg gtactgctgc ggtgctgacc ttggccgtgc tcttcctgac ggggagccag
     1441 gctcggcatt tctggcagca agatgaaccc ccccagagcc cctgggatcg agtgaaggac
     1501 ctggccactg tgtacgtgga tgtgctcaaa gacagcggca gagactatgt gtcccagttt
     1561 gaaggctccg ccttgggaaa acagctaaac ctaaagctcc ttgacaactg ggacagcgtg
     1621 acctccacct tcagcaagct gcgcgaacag ctcggccctg tgacccagga gttctgggat
     1681 aacctggaaa aggagacaga gggcctgagg caggagatga gcaaggatct ggaggaggtg
     1741 aaggccaagg tgcagcccta cctggacgac ttccagaaga agtggcagga ggagatggag
     1801 ctctaccgcc agaaggtgga gccgctgcgc gcagagctcc aagagggcgc gcgccagaag
     1861 ctgcacgagc tgcaagagta g
//