BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J54006 1 BBa_J54006 PoPS->deoR protein generator 2006-08-03T11:00:00Z 2015-08-31T03:54:05Z This part is based on part BBa_J54000 This is a RBS, CDS,Terminator construct used to take PoPS signal and produce deoR protein. false false _76_ 0 766 76 Not in stock false None. false Shogo Hamada component1893505 1 BBa_J54000 component1893500 1 BBa_B0030 component1893506 1 BBa_B0010 component1893508 1 BBa_B0012 annotation1893506 1 BBa_B0010 range1893506 1 792 871 annotation1893505 1 BBa_J54000 range1893505 1 22 783 annotation1893500 1 BBa_B0030 range1893500 1 1 15 annotation1893508 1 BBa_B0012 range1893508 1 880 920 BBa_J54000 1 BBa_J54000 deoR repressor protein 2006-08-03T11:00:00Z 2015-08-31T03:54:04Z It comes from Escherichia Coli. It is a negative DNA-binding transcriptional regulator that is involved in the negative regulation of the genes that encode the nucleotide catabolism (deoCABD, nupG, and tsx). The protein family is DeoR. RegulonDB http://regulondb.ccg.unam.mx:80/SearchListController?key_id_org=ECK12&object_id=DEOR&object_form=GN false false _76_ 0 766 76 Not in stock false I have not made any changes from wildtype. false Shogo Hamada annotation1893460 1 double TAA stop codons range1893460 1 757 762 annotation1893463 1 start codons range1893463 1 1 3 annotation1893453 1 deoR range1893453 1 1 762 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J54000_sequence 1 atggaaacacgtcgcgaagagcgtatcgggcagctgctgcaagaattaaaacgcagcgataagttacatcttaaagacgccgccgccctgcttggggtttcggagatgacgattcgtcgcgatctgaacaaccacagtgcgcccgtcgttttgctcggcggctatattgttctggaaccgcgcagtgccagccattacctgttaagcgatcaaaaatcccgcctggtggaagaaaaacgccgggcggcaaaactggctgcgacgctggtagaacccgatcagaccctcttttttgactgtggcaccaccacgccgtggattattgaagcgattgataatgaaatcccttttaccgccgtttgttattcgctaaatacctttctggcgctgaaagagaaaccccattgccgcgcgtttctttgcggtggtgaatttcacgccagcaacgccattttcaaacccatcgattttcagcaaacgctgaataatttttgcccggatatcgctttttattctgcggcgggcgtgcatgtcagtaaaggcgctacctgttttaatcttgaagagttgccggtaaaacactgggccatgtcgatggcgcaaaagcatgtgctggttgtcgaccacagtaaatttggcaaggtgcgtccggcgcgcatgggtgacctgaaacgctttgatattgtggtgagcgattgttgcccggaagatgagtatgtgaagtacgcgcagacgcagcgcattaagttgatgtattaataa BBa_B0030_sequence 1 attaaagaggagaaa BBa_J54006_sequence 1 attaaagaggagaaatactagatggaaacacgtcgcgaagagcgtatcgggcagctgctgcaagaattaaaacgcagcgataagttacatcttaaagacgccgccgccctgcttggggtttcggagatgacgattcgtcgcgatctgaacaaccacagtgcgcccgtcgttttgctcggcggctatattgttctggaaccgcgcagtgccagccattacctgttaagcgatcaaaaatcccgcctggtggaagaaaaacgccgggcggcaaaactggctgcgacgctggtagaacccgatcagaccctcttttttgactgtggcaccaccacgccgtggattattgaagcgattgataatgaaatcccttttaccgccgtttgttattcgctaaatacctttctggcgctgaaagagaaaccccattgccgcgcgtttctttgcggtggtgaatttcacgccagcaacgccattttcaaacccatcgattttcagcaaacgctgaataatttttgcccggatatcgctttttattctgcggcgggcgtgcatgtcagtaaaggcgctacctgttttaatcttgaagagttgccggtaaaacactgggccatgtcgatggcgcaaaagcatgtgctggttgtcgaccacagtaaatttggcaaggtgcgtccggcgcgcatgggtgacctgaaacgctttgatattgtggtgagcgattgttgcccggaagatgagtatgtgaagtacgcgcagacgcagcgcattaagttgatgtattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z