BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J54007 1 BBa_J54007 PoPs->cytR protein generator 2006-08-03T11:00:00Z 2015-08-31T03:54:05Z This device is based on BBa_J54001(cytR). This is a RBS.CDS.Terminator construct used to take in PoPs signal and produce the protein cytR. false false _76_ 0 762 76 Not in stock false None. false Yusaku Nakashima component1893471 1 BBa_B0010 component1893470 1 BBa_J54001 component1893466 1 BBa_B0032 component1893473 1 BBa_B0012 annotation1893473 1 BBa_B0012 range1893473 1 1145 1185 annotation1893470 1 BBa_J54001 range1893470 1 20 1048 annotation1893466 1 BBa_B0032 range1893466 1 1 13 annotation1893471 1 BBa_B0010 range1893471 1 1057 1136 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_J54001 1 BBa_J54001 cytR 2006-08-03T11:00:00Z 2015-08-31T03:54:05Z It comes from E.coli. regulonDB http://regulondb.ccg.unam.mx/RegulonControllerServlet?regulon_id=ECK12R016527 CytR transcriptional dual regulator,regulator for deo operon, udp, cdd, tsx, nupC, and nupG false false _76_ 0 762 76 Not in stock false I have not made any mutation to wildtype sequence. false Yusaku Nakashima annotation1893458 1 Double stop codon range1893458 1 1024 1029 annotation1893455 1 cytR range1893455 1 1 1029 annotation1893462 1 G to A range1893462 1 1 1 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0032_sequence 1 tcacacaggaaag BBa_J54001_sequence 1 atgaaagcgaagaagcaggaaactgccgcgaccatgaaagacgttgccctcaaggcaaaagtctctacagcgaccgtctcccgagcattaatgaatcccgataaagtctcccaggccacccgtaatcgggttgaaaaagcggcccgggaagtgggttatttaccgcagcctatggggcgcaacgtcaagcgtaatgaatcccgcaccattctggtgattgtcccggatatctgcgatcccttctttagcgaaattattcgcggtatcgaagttacggcggcaaatcacggatatctggtgctgattggcgactgtgcgcatcaaaatcagcaggaaaaaacctttatcgatttgatcatcaccaagcaaattgatggcatgttgttgctgggttcaaggctgccgtttgatgccagcattgaggaacagcgtaatctgccgccgatggtgatggcgaacgaatttgcaccggagctggagctgcctacagttcatatcgacaatctgaccgccgcatttgatgcagtaaattatttatatgagcaagggcataaacggattggctgtatagccggtcccgaagagatgccgctgtgtcactaccgcctgcaaggctatgttcaggcgctgcgtcgctgcggcattatggttgatccgcaatacatcgcccgtggcgacttcaccttcgaagccggaagcaaagcgatgcagcagctgcttgatcttccacaaccgcctactgctgtcttctgccatagcgatgtgatggcgctcggcgcactttctcaggcaaaacgccaggggctgaaagtcccggaagacctttccataatcggttttgataacatcgacctgacgcaattttgtgatccgccgctgacaaccatcgcgcagccgcgttacgaaatcggtcgggaagctatgctgttattgcttgatcaaatgcaggggcaacacgttggcagtggctctcgtttaatggactgcgaacttatcatccggggatcaacacgcgcgttaccttaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J54007_sequence 1 tcacacaggaaagtactagatgaaagcgaagaagcaggaaactgccgcgaccatgaaagacgttgccctcaaggcaaaagtctctacagcgaccgtctcccgagcattaatgaatcccgataaagtctcccaggccacccgtaatcgggttgaaaaagcggcccgggaagtgggttatttaccgcagcctatggggcgcaacgtcaagcgtaatgaatcccgcaccattctggtgattgtcccggatatctgcgatcccttctttagcgaaattattcgcggtatcgaagttacggcggcaaatcacggatatctggtgctgattggcgactgtgcgcatcaaaatcagcaggaaaaaacctttatcgatttgatcatcaccaagcaaattgatggcatgttgttgctgggttcaaggctgccgtttgatgccagcattgaggaacagcgtaatctgccgccgatggtgatggcgaacgaatttgcaccggagctggagctgcctacagttcatatcgacaatctgaccgccgcatttgatgcagtaaattatttatatgagcaagggcataaacggattggctgtatagccggtcccgaagagatgccgctgtgtcactaccgcctgcaaggctatgttcaggcgctgcgtcgctgcggcattatggttgatccgcaatacatcgcccgtggcgacttcaccttcgaagccggaagcaaagcgatgcagcagctgcttgatcttccacaaccgcctactgctgtcttctgccatagcgatgtgatggcgctcggcgcactttctcaggcaaaacgccaggggctgaaagtcccggaagacctttccataatcggttttgataacatcgacctgacgcaattttgtgatccgccgctgacaaccatcgcgcagccgcgttacgaaatcggtcgggaagctatgctgttattgcttgatcaaatgcaggggcaacacgttggcagtggctctcgtttaatggactgcgaacttatcatccggggatcaacacgcgcgttaccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z