BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_J54009
1
BBa_J54009
PoPs -> GalS protein generator
2006-08-03T11:00:00Z
2015-08-31T03:54:05Z
This device is based on BBa_J54002 (GalS)
This is RBS, CDS, teminator consturuct used to PoPS signal and produce the protein GalS
false
false
_76_
0
765
76
Not in stock
false
none
false
Shotaro Ayukawa
component1893486
1
BBa_B0011
component1893481
1
BBa_J54002
component1893482
1
BBa_B0012
component1893478
1
BBa_B0034
annotation1893486
1
BBa_B0011
range1893486
1
1120
1165
annotation1893481
1
BBa_J54002
range1893481
1
19
1062
annotation1893478
1
BBa_B0034
range1893478
1
1
12
annotation1893482
1
BBa_B0012
range1893482
1
1071
1111
BBa_J54002
1
BBa_J54002
galS
2006-08-03T11:00:00Z
2015-08-31T03:54:05Z
regulonDB
It comes from E.coli K12.
GalS is a isorepressor of galR.
false
false
_76_
0
765
76
Not in stock
false
I have not made mutation to wild type.
false
Shotaro Ayukawa
annotation1893456
1
GalS
range1893456
1
1
1044
annotation1893459
1
double TAA
range1893459
1
1039
1044
BBa_J54009_sequence
1
aaagaggagaaatactagatgatcaccattcgtgatgtagcgcgtcaggctggcgtctctgtggcaacggtttcccgggtgctcaataacagcacgctggtcagtgccgacacgcgtgaagcagtaatgaaagccgtgagtgagctggattatcggccaaacgccaatgcccaggcgctggcaactcaggttagcgacaccattggcgtggtggtgatggacgtttctgatgcgtttttcggcgcgctggtaaaagcggtggatctggtcgctcagcagcatcagaaatacgtgctaatcggcaatagctatcatgaagcggaaaaagagcgtcacgccattgaggtgttaattcgccagcgttgtaatgcgttgattgttcactcaaaagcattgagtgacgatgaactggcgcaatttatggataacattcccggtatggtgttaatcaaccgcgttgtgccggggtacgcccatcgttgcgtttgcctggataatctcagcggtgcccgaatggcgacgcgcatgttgctgaataacggtcatcaacgtattggttatctttcttccagccacggcattgaagatgacgccatgcgtaaagcaggctggatgagtgcgttgaaagagcaggatattattccgccggaaagctggattggcgctggtacgccggacatgccgggcggtgaggcggcgatggttgaactgctggggcgcaatctacaacttaccgctgtatttgcttataacgacaatatggccgctggcgcactgacagcattaaaagataatggcattgcgattccgttacatctctcaatcatcggtttcgatgatattcccatcgcccgttacaccgacccgcaattaacgaccgtgcgttatcccattgcttcaatggcgaaattagccaccgaactggccttgcagggggcagcaggcaatattgatcctcgtgccagccactgttttatgccgacgttagtgcgtcgccattctgtcgcaacacgccagaatgcggcggcgatcactaactcaacaaatcaggcgatgtaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J54002_sequence
1
atgatcaccattcgtgatgtagcgcgtcaggctggcgtctctgtggcaacggtttcccgggtgctcaataacagcacgctggtcagtgccgacacgcgtgaagcagtaatgaaagccgtgagtgagctggattatcggccaaacgccaatgcccaggcgctggcaactcaggttagcgacaccattggcgtggtggtgatggacgtttctgatgcgtttttcggcgcgctggtaaaagcggtggatctggtcgctcagcagcatcagaaatacgtgctaatcggcaatagctatcatgaagcggaaaaagagcgtcacgccattgaggtgttaattcgccagcgttgtaatgcgttgattgttcactcaaaagcattgagtgacgatgaactggcgcaatttatggataacattcccggtatggtgttaatcaaccgcgttgtgccggggtacgcccatcgttgcgtttgcctggataatctcagcggtgcccgaatggcgacgcgcatgttgctgaataacggtcatcaacgtattggttatctttcttccagccacggcattgaagatgacgccatgcgtaaagcaggctggatgagtgcgttgaaagagcaggatattattccgccggaaagctggattggcgctggtacgccggacatgccgggcggtgaggcggcgatggttgaactgctggggcgcaatctacaacttaccgctgtatttgcttataacgacaatatggccgctggcgcactgacagcattaaaagataatggcattgcgattccgttacatctctcaatcatcggtttcgatgatattcccatcgcccgttacaccgacccgcaattaacgaccgtgcgttatcccattgcttcaatggcgaaattagccaccgaactggccttgcagggggcagcaggcaatattgatcctcgtgccagccactgttttatgccgacgttagtgcgtcgccattctgtcgcaacacgccagaatgcggcggcgatcactaactcaacaaatcaggcgatgtaataa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z