LOCUS       BBa_J54232              1008 bp    DNA     linear       01-Jun-2024
DEFINITION  TetR_regulated(B-3)
ACCESSION   BBa_J54232
VERSION     BBa_J54232.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    97..103
                     /label=BglII
     misc_feature    72..96
                     /label=TetR
     promoter        72..109
                     /label=BBa_J54230
     misc_feature    118..122
                     /label=MulI
     misc_feature    118..122
                     /label=BBa_J54034
     RBS             131..145
                     /label=RBS-1\Strong
     RBS             138..142
                     /label=RBS
     misc_feature    131..145
                     /label=BBa_B0030
     RBS             131..145
                     /label=BBa_B0030
     CDS             152..871
                     /label=GFP protein
     CDS             152..871
                     /label=BBa_E0040
     stem_loop       891..934
                     /label=stem_loop
     misc_feature    880..959
                     /label=BBa_B0010
     terminator      880..959
                     /label=BBa_B0010
     stem_loop       975..994
                     /label=T7 TE
     misc_feature    1001..1001
                     /label=stop
     polyA_site      995..1008
                     /label=polya
     misc_feature    968..1008
                     /label=BBa_B0012
     terminator      968..1008
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcgtccc tatcagtgat agagatagat ctatcgtaat actagagcgc
      121 gttactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
      181 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      241 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      301 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      361 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      421 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      481 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      541 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      601 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      661 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      721 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      781 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      841 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      961 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//