LOCUS       BBa_J54251              1012 bp    DNA     linear       01-Jun-2024
DEFINITION  LacI_regulated(B-5)
ACCESSION   BBa_J54251
VERSION     BBa_J54251.1  
FEATURES             Location/Qualifiers
     misc_feature    1..5
                     /label=Sal
     misc_feature    1..5
                     /label=BBa_J54023
     misc_feature    20..26
                     /label=BamHI
     promoter        27..61
                     /label=lacq Promoter
     promoter        14..63
                     /label=BBa_J54200
     misc_feature    77..100
                     /label=LacI_Binding_Site
     misc_feature    101..106
                     /label=BglII
     promoter        72..113
                     /label=BBa_J54250
     misc_feature    122..126
                     /label=MulI
     misc_feature    122..126
                     /label=BBa_J54034
     RBS             135..149
                     /label=RBS-1\Strong
     RBS             142..146
                     /label=RBS
     misc_feature    135..149
                     /label=BBa_B0030
     RBS             135..149
                     /label=BBa_B0030
     CDS             156..875
                     /label=GFP protein
     CDS             156..875
                     /label=BBa_E0040
     stem_loop       895..938
                     /label=stem_loop
     misc_feature    884..963
                     /label=BBa_B0010
     terminator      884..963
                     /label=BBa_B0010
     stem_loop       979..998
                     /label=T7 TE
     misc_feature    1005..1005
                     /label=stop
     polyA_site      999..1012
                     /label=polya
     misc_feature    972..1012
                     /label=BBa_B0012
     terminator      972..1012
                     /label=BBa_B0012
ORIGIN
        1 gtcgatacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
       61 gcctactaga ggatcggaat tgtgagcgga taacaattcc agatctatcg taatactaga
      121 gcgcgttact agagattaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
      181 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      241 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      301 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
      361 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
      421 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      481 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
      541 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
      601 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
      661 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
      721 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      781 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
      841 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
      901 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      961 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//