LOCUS BBa_J5520 3445 bp DNA linear 17-May-2024 DEFINITION Arabinose -> LacI ts -| cI 434 ACCESSION BBa_J5520 VERSION BBa_J5520.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 misc_feature 1219..2589 /label=BBa_J06801 RBS 2598..2608 /label=RBS-4\Weaker RBS 2604..2607 /label=RBS misc_feature 2598..2608 /label=BBa_B0033 RBS 2598..2608 /label=BBa_B0033 CDS 2615..3283 /label=cI 434 misc_feature 3245..3283 /label=LVA misc_feature 2615..3283 /label=BBa_C0052 misc_feature 3284..3308 /label=Help:Barcodes CDS 2615..3283 /label=BBa_C0052 stem_loop 3328..3371 /label=stem_loop misc_feature 3317..3396 /label=BBa_B0010 terminator 3317..3396 /label=BBa_B0010 stem_loop 3412..3431 /label=T7 TE misc_feature 3438..3438 /label=stop polyA_site 3432..3445 /label=polya misc_feature 3405..3445 /label=BBa_B0012 terminator 3405..3445 /label=BBa_B0012 misc_feature 2598..3445 /label=BBa_P0352 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagtca cacaggacta ctagatgagt atttcttcca gggtaaaaag 2641 caaaagaatc cagcttggac ttaaccaggc tgaacttgct caaaaggtgg ggactaccca 2701 gcagtctata gagcagctcg aaaacggtaa aactaagcga ccacgctttt taccagaact 2761 tgcgtcagct cttggcgtaa gtgttgactg gctgctcaat ggcacctctg attcgaatgt 2821 tagatttgtt gggcacgttg agcccaaagg gaaatatcca ttgattagca tggttagagc 2881 tggttcgtgg tgtgaagctt gtgaacccta cgatatcaag gacattgatg aatggtatga 2941 cagtgacgtt aacttattag gcaatggatt ctggctgaag gttgaaggtg attccatgac 3001 ctcacctgta ggtcaaagca tccctgaagg tcatatggtg ttagtagata ctggacggga 3061 gccagtgaat ggaagccttg ttgtagccaa actgactgac gcgaacgaag caacattcaa 3121 gaaactggtc atagatggcg gtcagaagta cctgaaaggc ctgaatcctt catggcctat 3181 gactcctatc aacggaaact gcaagattat cggtgttgtc gtggaagcga gggtaaaatt 3241 cgtagctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 3301 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3361 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3421 tcgggtgggc ctttctgcgt ttata //