R6K

BBa_J61001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J61001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-07-30 11:00:00
Date modified: 2015-08-31 02:02:59

[R6K] Origin of replication



    • Details

    Types
    DnaRegion

    Roles
    DNA

    sequence_feature

    Sequences BBa_J61001_sequence (Version 1)

    Description

    R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature.

    {pSB1A2-Bca1011}

    Notes

    N/A

    Source


    PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI)

    Sub into pSB1A2-I13522 (EcoRI/SpeI)

    Product is Bca1011


    ca1011F Forward Biobricking of R6K

    GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg

    ca1011R Reverse biobricking of R6K

    ccgctactagtaGCAGTTCAACCTGTTGATAG



    Sequence Annotation Location Component / Role(s)
    R6K
    		27,406
    		feature/misc sequence_feature

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J61001/1
     

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