FRT

BBa_J61020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J61020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-09-20 11:00:00

Date modified: 2015-08-31 02:02:59

[FRT]

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_J61020_sequence (Version 1)

Description

Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.

Notes

N/A

Source

Extend ca1010F/R (73 bp, EcoRI/SpeI)

Sub into pSB1A2-I13522 (EcoRI/SpeI)

Product is pSB1A2-Bca1010

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ca1010F Forward (universal biobrick) EcoRI for FRT

GGACTgaattcgcggccgcttctagag

ca1010R Reverse SpeI oligo for FRT

cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J61020/1

Member of these Collections

• //function/recombination/flp
• iGEM Parts Registry