LOCUS BBa_J61031 10662 bp DNA linear 14-May-2024 DEFINITION Amplifiable BAC plasmid ACCESSION BBa_J61031 VERSION BBa_J61031.1 FEATURES Location/Qualifiers misc_feature 7864..8303 /label=pBR322 origin misc_feature 7691..8303 /label=pMB1 replication origin CDS 4891..5685 /label=kanamycin/neomycin resistance marker (NTP II marker) CDS 691..1662 /label=sopB CDS 1662..2837 /label=sopA CDS 3416..4171 /label=repE promoter 4753..4802 /label=kanR/neoR promoter misc_feature 5743..6126 /label=R6Kg origin stem_loop 6179..6222 /label=rrnB T1 terminator stem_loop 6188..6345 /label=rrnB terminator CDS 6451..6468 /label=6xHis tag CDS 6484..6513 /label=c myc tag promoter 6599..6627 /label=beta-lactamase (ampR) promoter CDS 6669..7529 /label=beta-lactamase ampicillin resistance marker CDS 8985..9698 /label=GFP reporter CDS 9815..10495 /label=mRFP stem_loop 10540..10583 /label=rrnB T1 terminator misc_feature 2..7 /label=SpeI misc_feature 9..16 /label=NotI misc_feature 2001..2006 /label=PstI misc_feature 3542..3547 /label=PstI misc_feature 5068..5073 /label=PstI misc_feature 6527..6532 /label=EcoRI misc_feature 8975..8980 /label=PstI misc_feature 145..618 /label=finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator; promoter 9735..9788 /label=BBa_R0040 Ptet promoter ORIGIN 1 gactagtagc ggccgcgatc ccaatccaca gcaggcatac aaccgcacac cgaggttact 61 ccgttctaca ggttacgacg acatgtcaat acttgccctt gacaggcatt gatggaatcg 121 tagtctcacg ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa 181 tcagaccgac aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga 241 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca 301 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg 361 acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc 421 gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata 481 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg 541 agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc 601 agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga 661 cagataaacg tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag 721 aatggcctca attttctcta tacactcagt tggaacacga gacctgtcca ggttaagcac 781 cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa 841 gctagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc 901 agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag 961 taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc 1021 accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg 1081 ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa 1141 ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata 1201 atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac 1261 acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc 1321 tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga 1381 agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga 1441 ccatacccga gaggtcttct caacactatc accccggagc acttcaagag taaacttcac 1501 atcccgacca catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc 1561 aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg 1621 agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt 1681 ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt 1741 ctccaggcac cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg 1801 atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc 1861 caggcatccc gaatttgctc ctccatccac ggggactgag agccattact attgctgtat 1921 ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga 1981 tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg 2041 ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc 2101 gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc 2161 atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca 2221 atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt 2281 attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt gtcttctgca 2341 tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta 2401 ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa 2461 actgaggttt tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg tggaaatacg 2521 tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt 2581 gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc 2641 cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca 2701 cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca 2761 atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg 2821 agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt 2881 tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata 2941 agagcaacac tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag 3001 cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa 3061 ctgttctgtc agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac 3121 tccaggtaga ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc 3181 ctcatcaatg atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag 3241 gaaatcaact gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa 3301 gtatgagaaa atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt 3361 aggtcagaac aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc 3421 ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt 3481 tctttttctc aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga 3541 cctgcaggaa gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac 3601 gctctatgat ccagtcgatt ttcagagaga cgatgcctga gccatccggc ttacgatact 3661 gacacaggga ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa 3721 gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga 3781 tatgtacact gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag 3841 attcatagcc tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc 3901 ccgcgaaact cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga 3961 atatttcagc atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc 4021 catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga 4081 gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt 4141 tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt 4201 ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca 4261 gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc 4321 atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc tctagctaga 4381 accagcatgg ataaaggcct acaaggcgct ctaaaaaaga agatctaaaa actataaaaa 4441 aaataattat aaaaatatcc ccgtggataa gtggataacc ccaagggaag ttttttcagg 4501 catcgtgtgt aagcagaata tataagtgct gttccctggt gcttcctcgc tcactcgagg 4561 gcttcgccct gtcgctcgac tgcggcgagc actactggct gtaaaaggac agaccacatc 4621 ataagcttga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 4681 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 4741 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 4801 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct 4861 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 4921 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 4981 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 5041 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 5101 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 5161 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcgcct tgctcctgcc 5221 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 5281 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 5341 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 5401 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgat ccatggcgat 5461 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcaa cgactgtggc 5521 cggctgggtg tggcggaccg ctatcaggac atagcgttgg atacccgtga tattgctgaa 5581 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 5641 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattgg agaccgcttg 5701 cgcaagatcc ggccacgatg cgtccggcgt agaggatctg aagatcagca gttcaacctg 5761 ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt taacgtacta 5821 agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct catggctaac 5881 gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa aattaatata 5941 aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa agaatatata 6001 aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac 6061 tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg 6121 acatgggaat tagccatggg cccgtgcgaa tcagaattag ttcaccgaca aacaacagat 6181 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc 6241 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt 6301 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca 6361 gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa 6421 aacagccaag ctggagaccg tttaaactca atgatgatga tgatgatggt cgacggcgct 6481 attcagatcc tcttctgaga tgagtttttg ttcgggccca agcttcgaat tcccatatgc 6541 ctgacggatg gcctttttgc gtttctacaa actctttttg tttatttttc taaatacatt 6601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 6661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 6721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 6781 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 6841 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 6901 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 6961 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 7021 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 7081 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 7141 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 7201 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 7261 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 7321 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 7381 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 7441 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 7501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 7561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 7621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 7681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 7741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 7801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 7861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 7921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 7981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 8041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 8101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 8161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 8221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 8281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 8341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 8401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 8461 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 8521 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 8581 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 8641 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 8701 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat 8761 caattcgcgc gcgaaggcga agcggcatgc ccttagctcc tgaaaatctc gccaagctag 8821 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga 8881 tggagttctg aggtcattac tggatctatc aacaggagtc caagctcagc taattaagct 8941 tattaagcta ctaaagcgta gttttcgtcg tttgctgcag gccttttgta tagttcatcc 9001 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt 9061 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg 9121 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca 9181 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc 9241 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca 9301 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac 9361 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt 9421 acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat 9481 cttgaaaagc attgaacacc ataagtcaaa gtagtgacaa gtgttggcca tggaacaggt 9541 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca 9601 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt 9661 gggacaactc cagtgaaaag ttcttctcct ttactcataa ataatgatga ctcctgaaaa 9721 taaacagagg atcttcccta tcagtgatag agattgacat ccctatcagt gatagagata 9781 ctgagcacta ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa 9841 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat 9901 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac 9961 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc 10021 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga 10081 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca 10141 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt 10201 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg 10261 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga 10321 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct 10381 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac 10441 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg 10501 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 10561 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 10621 actggctcac cttcgggtgg gcctttctgc gtttatacaa tt //