BBa_J61032 1 BBa_J61032 Alkaline phosphatase [phoA] 2006-11-10T12:00:00Z 2015-08-31T02:02:59Z pre-pcr ca1033F/ca1032R on Citrobacter gen (1500 bp) PCR ca1032F/R on above PCR (1500 bp, XbaI/EcoRI) Sub into pSB1A2-Bca1008 (XbaI/MfeI, 2077+935, large) Product is pSB1A2-Bca1032<br> ---- ca1032F Forward oligo to biobrick phoA of Citrobacter GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG ca1032R Reverse oligo to biobrick phoA of Citrobacter cagtaGAATTCccagaggcggcaTTACTTCAG ca1033F Forward constant oligo to clone phoA ttatcatcgctccgcatcctg Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites. false false _95_ 0 483 95 It's complicated false N/A false John Anderson BBa_J61032_sequence 1 atgttttttattttaactgtatttatacatggagaaaataaagtgaaacaaagcgctatgatttttgccttgctgcctttgctgttggctccggccagtcaggcggacaccacaacaatatccgtgctggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaatgccgcctctgggaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z