LOCUS       BBa_J61035              3539 bp    DNA     linear       17-May-2024
DEFINITION  EcoRI-GenR-XbaI
ACCESSION   BBa_J61035
VERSION     BBa_J61035.1  
FEATURES             Location/Qualifiers
     promoter        1859..1887
                     /label=beta-lactamase (ampR) promoter
     misc_feature    2090..2469
                     /label=R6K origin
     CDS             2535..3068
                     /label=Gentamycin resistance
     misc_feature    180..862
                     /label=ColE1 origin
     CDS             957..1817
                     /label=beta-lactamase (ampicillin resistance marker)
     misc_feature    2091..2474
                     /label=R6Kg origin
     misc_feature    183..802
                     /label=pBR322 origin
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    2058..2063
                     /label=EcoRI
     misc_feature    2514..2521
                     /label=NotI
     misc_feature    3533..3538
                     /label=XbaI
ORIGIN
        1 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1921 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1981 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2041 gctaaggatg atttctggaa ttctgattcg cacgggccca tggctaattc ccatgtcagc
     2101 cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc
     2161 gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata
     2221 tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata
     2281 ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta
     2341 gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt
     2401 acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg
     2461 ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat cttgcggccg
     2521 ccttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     2581 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     2641 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     2701 gcaatgccct gcctccggtg ctctccggag actgcgagat catagatata gatctcacta
     2761 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
     2821 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
     2881 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
     2941 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
     3001 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     3061 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     3121 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     3181 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     3241 ttacgaaccg aacaggctta tgtcaactgg gttcgtgggc gcctgagctt atgatgtggt
     3301 ctgtcctttt acagccagta gtgctcgccg cagtcgagcg acagggcgaa gccctcgagt
     3361 gagcgaggaa gcaccaggga acagcactta tatattctgc ttacacacga tgcctgaaaa
     3421 aacttccctt ggggttatcc acttatccac ggggatattt ttataattat tttttttata
     3481 gtttttagat cttctttttt agagcgcctt gtaggccttt atccatgctg gttctagag
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