LOCUS       BBa_J64714              1571 bp    DNA     linear       19-May-2024
DEFINITION  RHLR/RHLI Inducible (LasR/LasI repressible) positive feedback 
            production of C4 HSL
ACCESSION   BBa_J64714
VERSION     BBa_J64714.1  
FEATURES             Location/Qualifiers
     misc_feature    1..23
                     /label=RHLR/RHLI
     misc_feature    23..40
                     /label=LasR/LasI
     misc_feature    19..24
                     /label=-35
     misc_feature    42..47
                     /label=-10
     promoter        1..53
                     /label=BBa_J64800
     misc_feature    66..69
                     /label=conserved
     RBS             62..73
                     /label=BBa_B0034
     CDS             80..688
                     /label=BBa_J64718
     misc_feature    701..704
                     /label=conserved
     RBS             697..708
                     /label=BBa_B0034
     CDS             715..1434
                     /label=GFP protein
     CDS             715..1434
                     /label=BBa_E0040
     stem_loop       1454..1497
                     /label=stem_loop
     misc_feature    1443..1522
                     /label=BBa_B0010
     terminator      1443..1522
                     /label=BBa_B0010
     stem_loop       1538..1557
                     /label=T7 TE
     misc_feature    1564..1564
                     /label=stop
     polyA_site      1558..1571
                     /label=polya
     misc_feature    1531..1571
                     /label=BBa_B0012
     terminator      1531..1571
                     /label=BBa_B0012
ORIGIN
        1 tcctgtgaaa tctggcagtt acctgccagt tctggcaggt ctaaaaagtg ttctactaga
       61 gaaagaggag aaatactaga tgatcgaact gctgtccgaa tccctggaag gtctgtccgc
      121 tgctatgatc gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg
      181 ggacgttgtt tccacctccc gtgttcgtga ccaggagttc gaccagttcg accacccgca
      241 gacccgttac atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc
      301 gaccaccgac gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc
      361 gtccgacccg tccgtttggg aactgtcccg ttacgctgct tccgctgctg acgacccgca
      421 gctggctatg aaaatcttct ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc
      481 cgttgttgct gttaccacca ccgctatgga acgttacttc gttcgtaacg gtgttatcct
      541 ccagcgtctg ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta tctccttccc
      601 ggcttaccag gaacgtggtc tggaaatgct gctgcgttac cacccggaat ggctccaggg
      661 tgttccgctg tccatggctg tttaataata ctagagaaag aggagaaata ctagatgcgt
      721 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      781 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      841 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      901 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      961 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1021 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1081 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1141 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1201 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1261 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1321 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1381 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1441 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1501 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1561 ctgcgtttat a
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